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Immunosuppressive Drug Measurement by Liquid Chromatography Coupled to Tandem Mass Spectrometry: Interlaboratory Comparison in the Korean Clinical Laboratories

Kim HK, Park HD, Lee SG, Chae H, Song SH, Lee YW, Yun YM, Ahn S, Kim S, Lee SM, Lee SY, Chun S, on behalf of the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry

Background: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is increasingly used for immunosuppressive drug tests. However, most LC-MS/MS tests are laboratory-developed and their agreement is unknown in different Korean...
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Evaluation of antibacterial activity against Candida albicans according to the dosage of various denture cleansers

Bae CH, Lim YK, Kook JK, Son MK, Heo YR

PURPOSE: The purpose of this study is to compare the antibacterial activity of currently purchasable denture cleansers against Candida albicans. Materials and methods: This study used tablet-type denture cleansers, PolidentⓇ...
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Quantification of Thioguanine in DNA Using Liquid Chromatography-Tandem Mass Spectrometry for Routine Thiopurine Drug Monitoring in Patients With Pediatric Acute Lymphoblastic Leukemia

Choi R, Chun MR, Park J, Lee JW, Ju HY, Cho HW, Hyun JK, Koo HH, Yi ES, Lee SY

Background: We developed an assay to measure DNA-incorporated 6-thioguanine (DNATG) and validated its clinical applicability in Korean pediatric patients with acute lymphoblastic leukemia (ALL) in order to improve individualized thiopurine...
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Biomarkers and diagnostic tools for lung cancer

Lee SH, Lee EH, Kim EY, Choi YJ, Cha YJ, Chang YS

Lung cancer is the leading cause of cancer-related deaths in developing to advanced countries worldwide. The incidence rate of lung cancer in Korea has been increasing steadily since 1997. Statistics...
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A Sensitive and Specific Liquid ChromatographyTandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison With Immunoassays

Shin S, Oh H, Park HR, Joo EY, Lee SY

Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/ MS) assay for simultaneous measurement of salivary melatonin and...
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Prognostic Value of Serum Soluble Programmed Death-Ligand 1 and Dynamics During Chemotherapy in Advanced Gastric Cancer Patients

Park W, Bang JH, Nam AR, Jin MH, Seo H, Kim JM, Oh KS, Kim TY, Oh DY

Purpose The soluble form programmed death-ligand 1 (sPDL1) has immunosuppressive properties and is being studied as a candidate biomarker for immuno-oncology drug development. We measured the serum sPDL1 at pre-and...
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Comparison of the cyclic fatigue resistance of VDW.ROTATE, TruNatomy, 2Shape, and HyFlex CM nickel-titanium rotary files at body temperature

Gündoğar M, Uslu G, Özyürek T, Plotino G

Objectives: This study aims to compare the cyclic fatigue resistance of VDW.ROTATE, TruNatomy, 2Shape, and HyFlex CM nickel-titanium (NiTi) rotary files at body temperature. Materials and Methods: In total, 80 VDW.ROTATE...
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Recommendations for Liquid Chromatography-Mass Spectrometry in the Clinical Laboratory: Part III. Quality Assurance

Kim S, Lee SG, Moon SY, Park HD, Song SH, Lee K, Choi HJ, Lee SY, Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry

The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) in clinical laboratories is increasing and is likely to expand into even more clinical venues in the future. Mass spectrometry is the...
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Development of an Operational Plan for the Liquid Biopsy External Quality Assessment Program in Korea

Ahn S, Kim HK, Kim JH, Lee W, Chun S, Lee WG, Min WK

Background: Liquid biopsy is a useful assay for the diagnosis, treatment, and prognosis prediction of solid tumors and its clinical application is expanding. Therefore, the need for developing an External...
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Role of Liquid Biopsies in Colorectal Cancer

Kim SH, Keum B

  • KMID: 2503587
  • J Dig Cancer Rep.
  • 2020 Jun;8(1):56-60.
In recent years, liquid biopsy has received immense attention. Liquid biopsy is a minimally invasive method used for obtaining biological fluids including urine, pleural fluid and, mostly, peripheral blood. Liquid...
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Current status and future perspectives of liquid biopsy in non-small cell lung cancer

Chang S, Hur JY, Choi YL, Lee CH, Kim WS

With advances in target therapy, molecular analysis of tumors is routinely required for treatment decisions in patients with advanced non-small cell lung cancer (NSCLC). Liquid biopsy refers to the sampling...
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ESR1 Mutation Detection and Dynamicsin Meningeal Carcinomatosis in BreastCancer

Carausu M, Melaabi S, Pierga JY, Bidard FC, Cabel L

ESR1 mutation is frequently encountered in hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative metastatic breast cancer (MBC), especially after aromatase inhibitor (AI) therapy, as a mechanism of resistance to...
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Recommendations for Liquid Chromatography-Mass Spectrometry in the Clinical Laboratory: Part II. Method Validation

Moon SY, Choi HJ, Kim S, Lee K, Lee SG, Song SH, Lee SY, Park HD, Clinical Mass Spectrometry Research Committee of theKorean Society of Clinical Chemistry

The demand for obtaining test results using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for accurate diagnosis in the field of laboratory medicine is expected to increase, but it is still not...
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Comparison of the sodium content of Korean soup-based dishes prepared at home, restaurants, and schools in Seoul

Park Y, Yoon J, Chung SJ

Purpose: The aim of this study was to measure the sodium content of the solid and liquid components of soup-based dishes (SBD) including Korean soup, stew, noodle/dumplings, and watery kimchi...
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Liquid biopsy using extracellular vesicle–derived DNA in lung adenocarcinoma

Kim IA, Hur JY, Kim HJ, Lee SE, Kim WS, Lee KY

Blood liquid biopsy has emerged as a way of overcoming the clinical limitations of repeat biopsy by testing for the presence of acquired resistance mutations to therapeutic agents. Despite its...
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The Efficacy of 4 Liters of Clear Liquids for Small Bowel Preparation Prior to Video Capsule Endoscopy

Placone N, Bahar R, Mann S

Background/Aims: Optimal small bowel (SB) preparation for video capsule endoscopy (VCE) is controversial. Our study aimed to support the use of a specified volume of 4 liters of clear liquids...
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Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Shin DW, Sohn MJ, Cho CR, Koo Hw, Yoon SW

Objective : Fibrin sealants have been used for hemostasis, sealant for cerebrospinal fluid leakage, and adhesive barrier in neurosurgery. Further, as its clinical use and role of an effective drug...
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Recommendations for the Use of Liquid Chromatography-Mass Spectrometry in the Clinical Laboratory: Part I. Implementation and Management

Lee K, Moon SY, Kim S, Choi HJ, Lee SG, Park HD, Lee SY, Song SH, Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry

Many types of assays involving mass spectrometry have been developed and applied in clinics. However, mass spectrometry has not been widely implemented yet relative to other measurement methods, including biochemical...
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Subretinal Fluid Drainage through Original Breaks without Perfluorocarbon Liquid for Rhegmatogenous Retinal Detachment

Kim JH, Lee KW, Kang HG

PURPOSE: To evaluate the efficiency of a surgical method using original breaks to drain subretinal fluid without using retinotomy and perfluorocarbon liquid for patients with rhegmatogenous retinal detachment (RRD). METHODS: A...
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Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories: a Questionnaire Survey

Chae H, Cho SE, Park HD, Chun S, Lee YW, Yun YM, Song SH, Lee SG, Lee K, Song J, Lee SY, On behalf of the Clinical Mass Spectrometry Research Committee of Korean Society of Clinical Chemistry

BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey...
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