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Targeted Next-Generation Sequencing of Plasma CellFree DNA in Korean Patients with Hepatocellular Carcinoma

Chae H, Sung PS, Choi H, Kwon A, Kang D, Kim Y, Kim M, Yoon SK

Background: Hepatocellular carcinoma (HCC) is the second-most-common cause of cancer-related deaths worldwide, and an accurate and non-invasive biomarker for the early detection and monitoring of HCC is required. We assessed...
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Clinical Practice Guidelines for Prenatal Aneuploidy Screening and Diagnostic Testing from Korean Society of Maternal-Fetal Medicine:(1) Prenatal Aneuploidy Screening

Choe SA, Seol HJ, Kwon JY, Park CW, Kim M, Lee JY, Kim MA, Hwang HS, Na S, Shim JY, Kim K, Ryu HM

In 2019, the Korean Society of Maternal-Fetal Medicine developed the first Korean clinical practice guidelines for prenatal aneuploidy screening and diagnostic testing. These guidelines were developed by adapting established clinical...
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High Cell-Free DNA Levels in Cerebrospinal Fluid Predict Leptomeningeal Seeding of Hematologic Malignancy

Kim EY, Lee ST, Kim YS, Byun JM, Hong J, Shin DY, Koh Y, Kim I

Background and Purpose: The main difficulty when diagnosing leptomeningeal metastases (LMSs) is the low sensitivity of cytology. Cancer cells release cell-free DNA (cfDNA) during proliferation and apoptosis, and so we...
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Clinical significance of cell-free DNA as a prognostic biomarker in patients with diffuse large B-cell lymphoma

Eskandari , Manoochehrabadi S, Pashaiefar H, Zaimy MA, Ahmadvand

BACKGROUND: Cell-free DNA (cfDNA) has the potential to serve as a non-invasive prognostic biomarker in some types of neoplasia. The investigation of plasma concentration of cfDNA may reveal its use...
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Clinical Validation of Non-Invasive Prenatal Testing for Fetal Common Aneuploidies in 1,055 Korean Pregnant Women: a Single Center Experience

Lee DE, Kim H, Park J, Yun T, Park DY, Kim M, Ryu HM

BACKGROUND: Non-invasive prenatal testing (NIPT) using cell-free fetal DNA from maternal plasma for fetal aneuploidy identification is expanding worldwide. The objective of this study was to evaluate the clinical utility...
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Effects of Pre-analytical Variables on Cell-free DNA Extraction for Liquid Biopsy

Jeong TD, Kim MH, Park S, Chung HS, Lee JW, Chang JH, Huh J

BACKGROUND: Extraction of cell-free DNA (cfDNA) is a key step for determining the quality of cfDNA-related molecular diagnostics. We evaluated the effect of sample containers and sample storage conditions on...
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Detection of KRAS mutations in plasma cell-free DNA of colorectal cancer patients and comparison with cancer panel data for tissue samples of the same cancers

Min S, Shin S, Chung YJ

Robust identification of genetic alterations is important for the diagnosis and subsequent treatment of tumors. Screening for genetic alterations using tumor tissue samples may lead to biased interpretations because of...
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Plasma Cell-Free DNA as a Predictive Marker after Radiotherapy for Hepatocellular Carcinoma

Park S, Lee EJ, Rim CH, Seong J

PURPOSE: Cell-free DNA (cfDNA) is gaining attention as a novel biomarker for oncologic outcomes. We investigated the clinical significance of cfDNA in hepatocellular carcinoma (HCC) patients treated with radiotherapy (RT). MATERIALS...
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Cell-Free DNA in Oncology: Gearing up for Clinic

Ulrich BC, Paweletz C

In the past several years, interest in the clinical utility of cell-free DNA as a noninvasive cancer biomarker has grown rapidly. Success in the development of plasma genotyping assays and...
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Evaluation of Digital PCR as a Technique for Monitoring Acute Rejection in Kidney Transplantation

Lee H, Park YM, We YM, Han DJ, Seo JW, Moon H, Lee YH, Kim YG, Moon JY, Lee SH, Lee JK

Early detection and proper management of kidney rejection are crucial for the long-term health of a transplant recipient. Recipients are normally monitored by serum creatinine measurement and sometimes with graft...
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An Experience of Using the Harmony Test for Genomics-Based Non-Invasive Prenatal Testing

Seo DH, Cho SE, Kwak JR

Serological prenatal screening tests are widely used to detect fetal chromosomal abnormalities such as Down and Edward syndromes. Amniocentesis is conducted as a confirmatory test in the screening-positive case. After...
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Effect of blood pressure and glycemic control on the plasma cell-free DNA in hemodialysis patients

Jeong DW, Moon JY, Choi YW, Moon H, Kim K, Lee YH, Kim SY, Kim YG, Jeong KH, Lee SH

BACKGROUND: The plasma levels of cell-free DNA (cfDNA) are known to be elevated under inflammatory or apoptotic conditions. Increased cfDNA levels have been reported in hemodialysis (HD) patients. The aim...
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Clinical Study of Non-Invasive Prenatal Testing Using Next-Generation Sequencing

Seo DH, Cho DY, Kim J, Kim SY, Cho SE, Oh M

BACKGROUND: Serological prenatal screening tests are widely used to detect fetal chromosomal abnormalities such as Down and Edward syndromes. After determining the presence of fetal cell-free DNA in maternal blood,...
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Validation of fetus aneuploidy in 221 Korean clinical samples using noninvasive chromosome examination: Clinical laboratory improvement amendments-certified noninvasive prenatal test

Kim MJ, Kwon CH, Kim DI, Im HS, Park S, Kim JH, Bae JS, Lee M, Lee MS

PURPOSE: We developed and validated a fetal trisomy detection method for use as a noninvasive prenatal test (NIPT) including a Clinical Laboratory Improvement Amendments (CLIA)-certified bioinformatics pipeline on a cloud-based...
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