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Cloning, Expression, and Purification of Hyperthermophile α-Amylase from
Pyrococcus woesei -
Ghasemi A, Ghafourian S, Vafaei S, Mohebi R, Farzi M, Taherikalani M, Sadeghifard N
- KMID: 2518227
- Osong Public Health Res Perspect.
- 2015 Dec;6(6):336-340.
- doi: 10.1016/j.phrp.2015.10.003
Objectives In an attempt α-amylase gene from Pyrococcus woesei was amplified and cloned into a pTYB2 vector to generate the recombinant plasmid pTY- α-amylase. Methods Escherichia coli BL21 used as a host and... -
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