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Biochemical properties of full-length hepatitis C virus RNA-dependent RNA polymerase expressed in insect cells

Choi HB, Kim YG, Oh JW

  • KMID: 1119328
  • Exp Mol Med.
  • 2003 Dec;35(6):475-485.
The hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B protein, is the key viral enzyme responsible for replication of the HCV viral RNA genome. Although several full-length and truncated forms...
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Improved cycle sequencing of GC-rich DNA template

Choi JS, Kim JS, Joe CO, Kim SH, Ha KS, Park YM

  • KMID: 755022
  • Exp Mol Med.
  • 1999 Mar;31(1):20-24.
Even when DNA sequencing of purified DNA template failed under the optimal condition, it can be generally contributed to high GC content. GC-rich region of template causes a secondary structure to produce...
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