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Cellular characteristics of primary and immortal canine embryonic fibroblast cells

You S, Moon JH, Kim TK, Kim SC, Kim JW, Yoon DH, Kwak S, Hong KC, Choi YJ, Kim H

  • KMID: 1094076
  • Exp Mol Med.
  • 2004 Aug;36(4):325-335.
Using normal canine embryonic fibroblasts (CaEF) that were shown to be senescent at passages 7th-9th, we established two spontaneously immortalized CaEF cell lines (designated CGFR-Ca-1 and -2) from normal senescent...
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Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors

Kang SG, Lee EK, Schaus S, Henderson

  • KMID: 938883
  • Exp Mol Med.
  • 2001 Sep;33(3):174-178.
Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results. It is difficult to determine whether cell death is due to selection agents or gene transfection,...
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