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Detection of Mycobacterium leprae in Tissue and Blood by Polymerase Chain Reaction

Lee KS, Oh KY, Ryoo YW, Suh MH, Song JY

BACKGROUND: Methods to detect and quanitify Mycobacterium leprae(M. leprae)are needed for studies involving the epidemiology, pathogenesis, and chemotherapy of leprosy. Serological assays and skin tests lack the sensitivity and specificity...
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Random Amplified Polymorphic DNA for Classification and Identification of Dermatophytes

Lee YS, Yoo JI, Choi YH, Joo HY, Kim BS, Kim DH

  • KMID: 2307109
  • Korean J Med Mycol.
  • 1998 Dec;3(2):107-114.
BACKGROUND: Dermatophytoses are infections of keratinized tissues, that is, the epidermis, hair and nails, caused by a group of specialized fungi, the dermatophytes. Laboratory diagnoses of dermatophytes such as Tricophyton,...
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Analysis of Random Amplicied Polyorphic DNA (RAPD) for Sporothrix schenckii and Related Fungi

Lee JB, Kim M, Lee SC, Won YH, Kim YP

  • KMID: 2307099
  • Korean J Med Mycol.
  • 2000 Sep;5(3):113-119.
BACKGROUND: Sporotrichosis is a common deep cutaneous fungal disease caused by Sporothtix (S.) schenckii. The recent development of polymerase chain reaction (PCR) technology, in particular, arbitrarily primed PCR (AP-PCR) or...
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Determination of Proper Condition for Detection of Legionella pneumophila by Polymerase Chain Reaction

Jung DW, Lee HC, Chay KO, Chung JK, Ha DR, Ryu PY

  • KMID: 1807455
  • Chonnam Med J.
  • 2000 Jun;36(2):139-147.
BACKGROUND: Legionella pneumophila is the causative agent of Legionnaires' disease, a severe pneumonia, and the nonpneumonic syndrome Pontiac fever. Isolation of L. pneumophila from the patient specimens and environment is...
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High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens

Lim CS, Kim KH, Chung S, Kim YH, Choi JY, Kim IT

  • KMID: 1424268
  • Korean J Blood Transfus.
  • 2013 Apr;24(1):71-78.
BACKGROUND: Accurate typing of Duffy blood group is important because anti-Duffy antibodies cause hemolytic transfusion reaction and hemolytic disease of the newborn. The aim of this study was to evaluate...
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Association of the Single-Nucleotide Polymorphism and Haplotype of the Complement Receptor 1 Gene with Malaria

Lan Y, Wei CD, Chen WC, Wang JL, Wang CF, Pan GG, Wei YS, Nong LG

PURPOSE: Although the polymorphisms of erythrocyte complement receptor type 1 (CR1) in patients with malaria have been extensively studied, a question of whether the polymorphisms of CR1 are associated with...
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Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus

Oem JK, Kye SJ, Lee KN, Kim YJ, Park JY, Park JH, Joo YS, Song HJ

  • KMID: 1091201
  • J Vet Sci.
  • 2005 Sep;6(3):207-212.
One step TaqMan real-time reverse transcription polymerase chain reaction (R/T RT-PCR)using a set of primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for...
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TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus

Yang DK, Kweon CH, Kim BH, Lim SI, Kim SH, Kwon JH, Han HR

  • KMID: 1093630
  • J Vet Sci.
  • 2004 Dec;5(4):345-351.
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the...
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