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Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

Kim HK, Lyoo KS, Huynh TM, Moon HJ, Nguyen VG, Park BK

There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse...
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TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus

Yang DK, Kweon CH, Kim BH, Lim SI, Kim SH, Kwon JH, Han HR

  • KMID: 1093630
  • J Vet Sci.
  • 2004 Dec;5(4):345-351.
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the...
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