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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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Genetic Polymorphisms in VIR Genes among Indian Plasmodium vivax Populations

Gupta P, Pande V, Das A, Singh V

The vir genes are antigenic genes and are considered to be possible vaccine targets. Since India is highly endemic to Plasmodium vivax, we sequenced 5 different vir genes and investigated...
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Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi

Kong HH, Hwang MY, Kim HK, Chung DI

Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search,...
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Short-Cut Pathway to Synthesize Cellulose of Encysting Acanthamoeba

Moon EK, Kong HH

  • KMID: 1792132
  • Korean J Parasitol.
  • 2012 Dec;50(4):361-364.
The mature cyst of Acanthamoeba is highly resistant to various antibiotics and therapeutic agents. Cyst wall of Acanthamoeba are composed of cellulose, acid-resistant proteins, lipids, and unidentified materials. Because cellulose...
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Identification of Antigenic Proteins in Trichomonas vaginalis

Lee HY, Hyung S, Lee JW, Kim J, Shin MH, Ryu JS, Park SJ

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T....
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Rapid detection of Theileria sergenti by polymerase chain reaction

Choi EJ, Kang SW, Kweon CH, Jeong WS, Yoon YD, Song HJ

Four separate pairs of oligonucleotide primers within the coding region in a T. sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified...
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Trypanosome Glycosylphosphatidylinositol Biosynthesis

Hong Y, Kinoshita

Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known...
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Analysis of antigenic domain of GST fused major surface protein (p30) fragments of Toxoplasma gondii

Nam HW, Im KS, Baek EJ, Choi WY, Cho SY

Antigenic domain of major surface protein (p30) of Toxoplasma gondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were...
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Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China

Zhang Y, Lv Y, Zhang F, Zhang W, Wang J, Cui Y, Wang R, Jian F, Zhang L, Ning C

Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621...
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

Song KJ, Yang Z, Chong CK, Kim JS, Lee KC, Kim TS, Nam HW

  • KMID: 1792140
  • Korean J Parasitol.
  • 2013 Oct;51(5):503-510.
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can...
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Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China

Zheng G, Hu W, Liu Y, Luo Q, Tan L, Li G

The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban...
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Prevalence of Drug Resistance-Associated Gene Mutations in Plasmodium vivax in Central China

Lu F, Wang B, Cao J, Sattabongkot J, Zhou H, Zhu G, Kim K, Gao Q, Han ET

  • KMID: 1792136
  • Korean J Parasitol.
  • 2012 Dec;50(4):379-384.
Resistance of Plasmodium spp. to anti-malarial drugs is the primary obstacle in the fight against malaria, and molecular markers for the drug resistance have been applied as an adjunct in...
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Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients

Nam HW, Song KJ, Ahn HJ, Yang Z, Chong CK, Cho PY, Ahn SK, Kim TS

  • KMID: 1792161
  • Korean J Parasitol.
  • 2014 Apr;52(2):143-149.
To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic...
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PCR Diagnosis of Entamoeba histolytica Cysts in Stool Samples

Moon JH, Cho SH, Yu JR, Lee WJ, Cheun HI

Amebiasis is a protozoan disease caused by Entamoeba histolytica and a potential health threat in areas where sanitation and hygiene are inappropriate. Highly sensitive PCR methods for detection of E....
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Plasmodium falciparum Genotype Diversity in Artemisinin Derivatives Treatment Failure Patients along the Thai-Myanmar Border

Congpuong , Hoonchaiyapoom T, Inorn K

Genetic characteristics of Plasmodium falciparum may play a role in the treatment outcome of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1), msp-2,...
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Presence of Cryptosporidium spp. and Giardia duodenalis in Drinking Water Samples in the North of Portugal

Almeida A, Moreira MJ, Soares S, Delgado MD, Figueiredo J, Silva E, Castro A, Cosa JM

Cryptosporidium and Giardia are 2 protozoan parasites responsible for waterborne diseases outbreaks worldwide. In order to assess the prevalence of these protozoans in drinking water samples in the northern part...
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Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea

Kim HY, Kim YA, Lee HS, Rhie HG, Cho SH, Yu JR, Lee SE

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2...
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A Novel Polyclonal Antiserum against Toxoplasma gondii Sodium Hydrogen Exchanger 1

Xiao B, Kuang Z, Zhan Y, Chen D, Gao Y, Li M, Luo S, Hao W

The sodium hydrogen exchanger 1 (NHE1), which functions in maintaining the ratio of Na+ and H+ ions, is widely distributed in cell plasma membranes. It plays a prominent role in...
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An Autochthonous Case of Canine Visceral Leishmaniasis in Korea

Bhang DH, Choi US, Kim HJ, Cho KO, Shin SS, Youn HJ, Hwang CY, Youn HY

  • KMID: 1792146
  • Korean J Parasitol.
  • 2013 Oct;51(5):545-549.
A 12-year-old spayed female mixed-bred dog presented with nasal bleeding of 2 days duration and a skin nodule in the left flank. No abnormalities were found in coagulation profiles and...
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Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri

Jeong SR, Kang SY, Lee SC, Song KJ, Im KI, Shin HJ

The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed...
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