- The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells
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Lv J, Wu S, Wei R, Li Y, Jin J, Mu Y, Zhang Y, Kong Q, Weng X, Liu Z
- KMID: 2448707
- J Vet Sci.
- 2019 May;20(3):e23.
- doi: 10.4142/jvs.2019.20.e23
The clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a versatile genome editing tool with high efficiency. A guide sequence of 20 nucleotides (nt) is commonly... -
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- Exploring differentially expressed genes related to metabolism by RNASeq in porcine embryonic fibroblast after insulin treatment
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Liang Y, Wang J, Li X, Wu S, Jiang C, Wang Y, Li X, Liu ZH, Mu Y
- KMID: 2536697
- J Vet Sci.
- 2022 Nov;23(6):e90.
- doi: 10.4142/jvs.22088
Background: Insulin regulates glucose homeostasis and has important effects on metabolism, cell growth, and differentiation. Depending on the cell type and physiological context, insulin signal has specific pathways and biological... -
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