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Clinical and Technical Aspects in Free Cortisol Measurement

Choi MH

Accurate measurement of cortisol is critical in adrenal insufficiency as it reduces the risk associated with misdiagnosis and supports the optimization of stress dose. Comprehensive assays have been developed to...
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Serum Ceruloplasmin as a Potential Clinical Biomarker in Atopic Dermatitis

Bae Y, Kim SJ

Background: Although the discovery of new biomarkers in atopic dermatitis (AD) is challenging, it is valuable in diagnosis, assessment of severity, and evaluation of treatment response. Objective: This study was designed...
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Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide

Moghrovyan A, Parseghyan L, Sevoyan G, Darbinyan A, Sahakyan N, Gaboyan M, Karabekian Z, Voskanyan A

Background: Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian...
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Proteomic identification of arginine-methylated proteins in colon cancer cells and comparison of messenger RNA expression between colorectal cancer and adjacent normal tissues

Lim Y, Gang DY, Lee WY, Yun SH, Cho YB, Huh JW, Park YA, Kim HC

Purpose: Identification of type I protein arginine methyltransferase (PRMT) substrates and their functional significance during tumorigenesis is becoming more important. The present study aimed to identify target substrates for type...
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Low Exposure to Direct Oral Anticoagulants Is Associated with Ischemic Stroke and Its Severity

Rizos T, Meid AD, Huppertz A, Dumschat C, Purrucker J, Foerster KI, Burhenne J, Czock D, Jenetzky E, Ringleb PA, Haefeli WE

Background and purpose In acute stroke patients, plasma concentrations of direct oral anticoagulants (DOAC) at hospital admission only poorly mirror DOAC exposure or the coagulation status at the time of...
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Anti-nociceptive and anti-inflammatory activities of the essential oil isolated from Cupressus arizonica Greene fruits

Fakhri S, Jafarian S, Majnooni MB, Farzaei MH, Mohammadi-Noori E, Khan H

Background: Cupressus arizonica Greene is a coniferous tree with great importance in fragrance and pharmaceutical industries. Essential oils from C. arizonica (EC) have shown potential antioxidant, and anti-microbial activities. This...
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Profiling of RNA-binding Proteins Interacting With Glucagon and Adipokinetic Hormone mRNAs

Ko S, Yeom E, Chun YL, Mun H, Howard-McGuire M, Millison NT, Jung J, Lee KP, Lee C, Lee KS, Delaney JR, Yoon JH

Objective: Glucagon in mammals and its homolog (adipokinetic hormone [AKH] in Drosophila melanogaster) are peptide hormones which regulate lipid metabolism by breaking down triglycerides. Although regulatory mechanisms of glucagon and...
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Determination of candesartan or olmesartan in hypertensive patient plasma using UPLC-MS/MS

Jeong HC, Seo YH, Gu N, Rhee MY, Shin KH

Candesartan and olmesartan are angiotensin II receptor blockers (ARBs) used for the treatment of hypertension and heart failure. Quantitation methods for candesartan and olmesartan were developed using ultra-high performance liquid...
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A validated simple LC-MS/MS method for quantifying trimethylamine N-oxide (TMAO) using a surrogate matrix and its clinical application

Li Y, Kang J, Lee Y, Chung JY, Cho JY

Trimethylamine N-oxide (TMAO) is a small molecular amine oxide generated from dietary choline and carnitine through intestinal microbial metabolism. Recently, TMAO has attracted much public attention as its role...
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Systematic Review of Recent Lipidomics Approaches Toward Inflammatory Bowel Disease

Lee EG, Yoon YC, Yoon J, Lee SJ, Oh YK, Kwon SW

Researchers have endeavored to identify the etiology of inflammatory bowel diseases, including Crohn’s disease and ulcerative colitis. Though the pathogenesis of inflammatory bowel diseases remains unknown, dysregulation of the immune...
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The possibility of low isomerization of β-lapachone in the human body

Lee KM, Gwon MR, Lee HW, Seong SJ, Yoon YR

β-Lapachone has been reported to have anticancer and various other therapeutic effects, but is limited in clinical applications by its low bioavailability. pH-Dependent isomerization can be suggested as one plausible...
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Validation of LC-MS/MS method for determination of rosuvastatin concentration in human blood collected by volumetric absorptive microsampling (VAMS)

Moon SJ, Lee SE, Kwak YG, Kim MG

In light of the shift toward patient-centric clinical trials, a measure of simplifying blood collection process and minimizing the volume of blood samples is on the rise. Volumetric absorptive microsampling...
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Immunosuppressive Drug Measurement by Liquid Chromatography Coupled to Tandem Mass Spectrometry: Interlaboratory Comparison in the Korean Clinical Laboratories

Kim HK, Park HD, Lee SG, Chae H, Song SH, Lee YW, Yun YM, Ahn S, Kim S, Lee SM, Lee SY, Chun S, on behalf of the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry

Background: Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is increasingly used for immunosuppressive drug tests. However, most LC-MS/MS tests are laboratory-developed and their agreement is unknown in different Korean...
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Quantification of Thioguanine in DNA Using Liquid Chromatography-Tandem Mass Spectrometry for Routine Thiopurine Drug Monitoring in Patients With Pediatric Acute Lymphoblastic Leukemia

Choi R, Chun MR, Park J, Lee JW, Ju HY, Cho HW, Hyun JK, Koo HH, Yi ES, Lee SY

Background: We developed an assay to measure DNA-incorporated 6-thioguanine (DNATG) and validated its clinical applicability in Korean pediatric patients with acute lymphoblastic leukemia (ALL) in order to improve individualized thiopurine...
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Performance Evaluation of VITEK MS for the Identification of a Wide Spectrum of Clinically Relevant Filamentous Fungi Using a Korean Collection

Shin JH, Kim SH, Lee D, Lee SY, Chun S, Lee JH, Won EJ, Choi HJ, Choi HW, Kee SJ, Shin MG, Shin JH

The correct identification of filamentous fungi is challenging. We evaluated the performance of the VITEK MS v3.0 system (bioMérieux, Marcy-l’Étoile, France) for the identification of a wide spectrum of clinically...
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A Case of Infective Endocarditis Caused by Kytococcus schroeteri

Yun J, Kim TS, Park H, Park JH

Kytococcus spp. is a skin bacterial flora that rarely causes human infections.K. schroeteri has been reported to cause infective endocarditis and bloodstream infections in patients with prosthetic valves and leukemia,...
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A Sensitive and Specific Liquid ChromatographyTandem Mass Spectrometry Assay for Simultaneous Quantification of Salivary Melatonin and Cortisol: Development and Comparison With Immunoassays

Shin S, Oh H, Park HR, Joo EY, Lee SY

Melatonin and cortisol are clinically important for diagnosing sleep and mood disorders. We developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/ MS) assay for simultaneous measurement of salivary melatonin and...
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Alterations of Spermatozoa Proteomic Profile in Men with Hodgkin’s Disease Prior to Cancer Therapy

Martins AD, Agarwal A, Baskaran S, Pushparaj PN, Ahmad G, Panner Selvam MK

Purpose: Hodgkin’s disease (HD) is a type of cancer affecting men in the reproductive age with potential consequences on their fertility status. This study aims to analyze sperm parameters, alterations...
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Evaluation of the Performance of ASTA MicroIDSys, a Novel Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry System, in Identification of Bacterial Clinical Isolates

Liu C, Lee E, Kim D, Jeong SH

ackground: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the...
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The Discovery of Clinically Applicable Biomarkers for Bipolar Disorder: A Review of Candidate and Proteomic Approaches

Muneer A

Bipolar disorder (BD) is a severe psychiatric condition which affects innumerable people across the globe. The etiopathogenesis of BD is multi-faceted with genetic, environmental and psychosocial factors playing a role....
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