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Improved Transformation of the Filamentous Fungus Aspergillus niger Using Agrobacterium tumefaciens

Park SM

  • KMID: 2312346
  • Mycobiology.
  • 2001 Sep;29(3):132-134.
Since it is known that Agrobacterium tumefaciens, which has long been used to transform plants, can transfer the T-DNA to yeast Saccharomyces cerevisiae during tumourigenesis, a variety of fungi were...
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Establishment of Immotalized Human Gingival Fibroblast Cell Lines

Song JB, Kim HA, Hyun HN, Kim EC, You HK, Shin HS

Human gingival fibroblasts have proven to useful as a species specific cell culture system in various system on periodontal disease and regeneration. However, their use is limited, since they are...
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Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes

Kim JK, Park YJ, Kong WS, Kang HW

  • KMID: 2312600
  • Mycobiology.
  • 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using...
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Improvement of Transformation System in Filamentous Fungus Aspergillus oryzae

Lee JW, Yun CD, Hahm YT

  • KMID: 2243203
  • Korean J Med Mycol.
  • 2001 Mar;6(1):1-8.
Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. A. oryzae is an important microorganism for industrial production of enzymes and fermented food products. It secrets large...
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Expression of pancreatic and duodenal homeobox1 (PDX1) protein in the interior and exterior regions of the intestine, revealed by development and analysis of Pdx1 knockout mice

Hashimoto , Kamisako T, Kagawa T, Haraguchi , Yagoto M, Takahashi , Kawai K, Suemizu H

We developed pancreatic and duodenal homeobox1 (Pdx1) knockout mice to improve a compensatory hyperinsulinemia, which was induced by hyperplasia in the beta cells or Langerhans' islands, as the diabetic model...
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A simple human immunodeficiency virus vector system for selective infection of CD4(+) cells and inducible expression of foreign genes

Kim YS

  • KMID: 2045605
  • Exp Mol Med.
  • 1997 Jun;29(2):103-110.
The alteration of T lymphocyte functions as a consequence of human immunodeficiency virus (HIV) infection is a potential target for the genetic treatment of the acquired immunodeficiency syndrome (AIDS). One...
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