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DNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR

Hawash Y

  • KMID: 1792180
  • Korean J Parasitol.
  • 2014 Jun;52(3):263-271.
PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles...
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Internal Amplification Control for a Cryptosporidium Diagnostic PCR: Construction and Clinical Evaluation

Hawash Y, Ghonaim MM, Al-Hazmi A

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it...
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Prevalence of Cryptosporidium-Associated Diarrhea in a High Altitude-Community of Saudi Arabia Detected by Conventional and Molecular Methods

Hawash Y, Dorgham LS, Al-Hazmi A, Al-Ghamdi

Cryptosporidium diarrhea represents a relevant clinical problem in developing countries. In Al-Taif, a city of Saudi Arabia that lies at an altitude of an around 2 km above the sea...
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Malaria Prevalence in a Low Transmission Area, Jazan District of Southwestern Saudi Arabia

Hawash Y, Ismail K, Alsharif K, Alsanie W

Detailed description of malaria in low transmission areas is crucial for elimination. The current study aimed to provide a comprehensive description for malaria transmission in Jazan, a low transmission district,...
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An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting

Hawash Y, Ghonaim MM, Al-Shehri

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round...
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