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Evaluation of alpha-Tubulin as an Antigenic and Molecular Probe to Detect Giardia lamblia

Kim J, Shin MH, Song KJ, Park SJ

The alpha/beta-tubulin heterodimer is the basic subunit of microtubules in eukaryotes. Polyclonal antibodies specific to recombinant alpha-tubulin of Giardia lamblia were made, and found effective as a probe to specifically...
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Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea

Park SJ, Yong TS, Yang HW, Lee DH, Lee K

Inoculating of human fecal cysts to suckling Mongolian gerbils, two Giardia lamblia isolates, K1 and K2, were established as axenic cultures. Using this in vitro culture, both isolates were characterized...
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Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification

Li J, Wang P, Zhang A, Zhang P, Alsarakibi M, Li G

Giardia lamblia is recognized as one of the most prevalent parasites in dogs. The present study aimed to establish a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection...
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An Improved PCR-RFLP Assay for Detection and Genotyping of Asymptomatic Giardia lamblia Infection in a Resource-Poor Setting

Hawash Y, Ghonaim MM, Al-Shehri

Laboratory workers, in resource-poor countries, still consider PCR detection of Giardia lamblia more costly and more time-consuming than the classical parasitological techniques. Based on 2 published primers, an in-house one-round...
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