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An improved method for constructing a full-length enriched cDNA library using small amounts of total RNA as a starting material

Oh JH, Kim YS, Kim NS

  • KMID: 1119343
  • Exp Mol Med.
  • 2003 Dec;35(6):586-590.
We have developed an improved method for constructing a full-length cDNA library using small quantity of material by modifying the original oligo-capping method. In our devised method, total RNAs are...
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Differential inhibition of endothelial cell proliferation and migration by urokinase subdomains: amino-terminal fragment and kringle domain

Kim KS, Hong YK, Lee Y, Shin JY, Chang SI, Chung SI, Joe YA

  • KMID: 1119342
  • Exp Mol Med.
  • 2003 Dec;35(6):578-585.
The serine protease urokinase-type plasminogen activator (uPA) is implicated in pericellular proteolysis in a variety of physiological and pathological processes including angiogenesis and tumor metastasis. The kringle domain of uPA...
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Targeting efficiency of a-1,3-galactosyl transferase gene in pig fetal fibroblast cells

Jin DI, Lee SH, Choi JH, Lee JS, Lee JE, Park KW, Seo JS

  • KMID: 1119341
  • Exp Mol Med.
  • 2003 Dec;35(6):572-577.
Animal cloning technology with somatic cells provides an alternative tool to conventional methods for producing transgenic animals. Gene targeting in animals is made feasible using somatic cells with homologous recombination...
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Role of crosslinked protein in lung injury following total body irradiation and bone marrow transplantation

Lee SY, Kim YJ, Kim YJ

  • KMID: 1119340
  • Exp Mol Med.
  • 2003 Dec;35(6):565-571.
The aberrant protein crosslinks formation during lung injury as results total body irradiation (TBI) and bone marrow transplantation (BMT) therapy has been examined as apossible contributory factor in organ or...
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Protein kinase CK2 phosphorylates and interacts with deoxyhypusine synthase in HeLa cells

Kang KR, Chung SI

  • KMID: 1119339
  • Exp Mol Med.
  • 2003 Dec;35(6):556-564.
Deoxyhypusine is a modified lysine and formed posttranslationally to be the eukaryotic initiation factor eIF5A by deoxyhypusine synthase, employing spermidine as butylamine donor. Subsequent hydroxylation of this deoxyhypusine-containing intermediate completes...
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Vitamin D receptor gene polymorphisms in breast cancer

Buyru N, Tezol A, Yosunkaya-Fenerci E, Dalay N

  • KMID: 1119338
  • Exp Mol Med.
  • 2003 Dec;35(6):550-555.
Breast cancer is the leading cause of cancer death among women around the world and its incidence is annually increasing. The vitamin D receptor (VDR) gene is a member of...
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Angiotensin converting enzyme I/D, angiotensinogen T174M-M235T and angiotensin II type 1 receptor A1166C gene polymorphisms in Turkish hypertensive patients

Agachan B, Isbir T, Yilmaz H, Akoglu E

  • KMID: 1119337
  • Exp Mol Med.
  • 2003 Dec;35(6):545-549.
Essential hypertension is a multifactorial disease in which genetic and enviromental factors play an important role. These factors differ in each population. As there are no existing data for the...
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Dysferlin in a hyperCKaemic patient with caveolin 3 mutation and in C2C12 cells after p38 MAP kinase inhibition

Capanni C, Sabatelli , Mattioli E, Ognibene A, Columbaro , Lattanzi G, Merlini L, Minetti C, Maraldi NM, Squarzoni

  • KMID: 1119336
  • Exp Mol Med.
  • 2003 Dec;35(6):538-544.
Dysferlin is a plasma membrane protein of skeletal muscle whose deficiency causes Miyoshi myopathy, limb girdle muscular dystrophy 2B and distal anterior compartment myopathy. Recent studies have reported that dysferlin...
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Melting of complexes of DNA-cis-DDP in acidic environment

Dalyan YB, Haroutiunian TS, Haroutiunian SG, Vardevanyan PO

  • KMID: 1119335
  • Exp Mol Med.
  • 2003 Dec;35(6):534-537.
The peculiarities of helix-coil transition of DNA at complex-formation with cis-diamminedichlorplatinum (II) (cis-DDP) have been investigated in acidic environment by UV absorbance technique. It is shown that observed features of...
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The binding of ethidium bromide with DNA: interaction with single- and double-stranded structures

Vardevanyan PO, Antonyan A, Parsadanyan MA, Davtyan HG, Karapetyan AT

  • KMID: 1119334
  • Exp Mol Med.
  • 2003 Dec;35(6):527-533.
The pH-induced helix-coil transition of DNA and its complexes with EtBr is carried out at acidic pH in a wide interval of change of concentration ratio of EtBr/DNA. The binding...
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Atrophy of brown adipocytes in the adult mouse causes transformation into white adipocyte-like cells

Kim DW, Kim BS, Kwon HS, Kim CG, Lee HW, Choi WH, Kim CG

  • KMID: 1119333
  • Exp Mol Med.
  • 2003 Dec;35(6):518-526.
Adipose tissue is an important endocrine regulator of glucose metabolism and energy homeostasis. Researches have focused on this tissue not only as a target for pharmacotherapy of obesity and insulin...
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Constitutive expression of 4-1BB on T cells enhances CD4+ T cell responses

Kim J, Choi SP, La S, Seo JS, Kim KK, Nam SH, Kwon B

  • KMID: 1119332
  • Exp Mol Med.
  • 2003 Dec;35(6):509-517.
4-1BB, a transmembrane molecule, member of the tumor necrosis factor receptor superfamily, is an important costimulatory molecule in the immune response, plays a key role in the clonal expansion and...
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High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases

Jung HW, La SJ, Kim JY, Heo SK, Kim JY, Wang S, Kim KK, Lee KM, Cho HR, Lee HW, Kwon B, Kim BS, Kwon BS

  • KMID: 1119331
  • Exp Mol Med.
  • 2003 Dec;35(6):501-508.
Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell...
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Adenoviral mediated hepatocyte growth factor gene attenuates hyperglycemia and beta cell destruction in overt diabetic mice

Park MK, Kim DK, Lee HJ

  • KMID: 1119330
  • Exp Mol Med.
  • 2003 Dec;35(6):494-500.
Hepatocyte growth factor (HGF) is a potent mitogen and promoter of proliferation of insulin producing beta cells of pancreatic islets. To study the role of HGF, an adenoviral vector carrying...
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Nonspecific association of 2',3'-cyclic nucleotide 3'-phosphodiesterase with the rat forebrain postsynaptic density fraction

Cho SJ, Jung JS, Shin SC, Jin IN, Ko BH, Kim Kwon Y, Suh-Kim H, Moon IS

  • KMID: 1119329
  • Exp Mol Med.
  • 2003 Dec;35(6):486-493.
The 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNP), a protein of unknown function in vivo, is abundantly expressed in myelinating glia in two isoforms, CNP1 and CNP2. In this study, immunoblot analysis showed...
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Biochemical properties of full-length hepatitis C virus RNA-dependent RNA polymerase expressed in insect cells

Choi HB, Kim YG, Oh JW

  • KMID: 1119328
  • Exp Mol Med.
  • 2003 Dec;35(6):475-485.
The hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B protein, is the key viral enzyme responsible for replication of the HCV viral RNA genome. Although several full-length and truncated forms...
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Resveratrol derivatives potently induce apoptosis in human promyelocytic leukemia cells

Kang JH, Park YH, Choi SW, Yang EK, Lee WJ

  • KMID: 1119327
  • Exp Mol Med.
  • 2003 Dec;35(6):467-474.
Resveratrol has been shown to possess antioxidant and anticancer activities, but little is known on the effect of resveratrol derivatives. Recently we have isolated resveratrol and its dimers and trimers...
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