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A decrease in the expression of CD63 tetraspanin protein elevates invasive potential of human melanoma cells

Jang HI, Lee H

  • KMID: 1109322
  • Exp Mol Med.
  • 2003 Aug;35(4):317-323.
CD63, which belongs to the tetraspanin membrane proteins, has been proposed to play an important role in inhibiting melanoma metastasis. To determine whether reduction of CD63 expression, which frequently occurs...
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Detection of antibodies against glucose 6-phosphate isomerase in synovial fluid of rheumatoid arthritis using surface plasmon resonance (BIAcore)

Kim JY, Lee MH, Jung KI, Na HY, Cha HS, Ko EM, Kim TJ

  • KMID: 1109321
  • Exp Mol Med.
  • 2003 Aug;35(4):310-316.
We have used a surface plasmon resonance biosensor (SPR, BIACORE 2000) to detect antibodies against glucose 6-phosphate isomerase (GPI) in synovial fluids of rheumatoid arthritis (RA) and osteoarthritis (OA). Recombinant...
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Activation of calcium signaling by hepatitis B virus-X protein in liver cells

Oh JC, Jeong DL, Kim IK, Oh SH

  • KMID: 1109320
  • Exp Mol Med.
  • 2003 Aug;35(4):301-309.
Hepatitis B virus x gene product (HBx) is known to be a transactivator of transcriptional elements that regulate the expression of a variety of genes associated with the growth, differentiation,...
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Cytochrome C-dependent Fas-independent apoptotic pathway in HeLa cells induced by delta12-prostaglandin J2

Kim BE, Roh SR, Kim JW, Jeong SW, Kim IK

  • KMID: 1109319
  • Exp Mol Med.
  • 2003 Aug;35(4):293-300.
Cyclopentenone prostaglandins (PGs) have antiproliferative activity on various tumor cell growth in vitro. Particularly, 9-deoxy-(9,12)-13,14-dihydro PGD2( delta12-PGJ2) was reported for its antineoplastic and apoptotic effects on various cancer cells, but...
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Molecular identification of Ca(2+)channels in human sperm

Park JY, Ahn HJ, Gu JG, Lee KH, Kim JS, Kang HW, Lee JH

  • KMID: 1109318
  • Exp Mol Med.
  • 2003 Aug;35(4):285-292.
The acrosome reaction is a Ca(2+)-dependent exocytotic process that is a prerequisite step for fertilization. External calcium entry through voltage-activated Ca(2+)channels is known to be essential in inducing the acrosome...
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Lipid rafts are important for the association of RANK and TRAF6

Ha H, Kwak HB, Le SW, Kim HH, Lee ZH

  • KMID: 1109317
  • Exp Mol Med.
  • 2003 Aug;35(4):279-284.
Rafts, cholesterol- and sphingolipid-rich membrane microdomains, have been shown to play an important role in immune cell activation. More recently rafts were implicated in the signal transduction by members of...
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N-Acetyl-beta-D-glucosaminidase in acute myocardial infarction

Iqbal MP, Kazmi KA, Jafri HR, Mehboobali N

  • KMID: 1109316
  • Exp Mol Med.
  • 2003 Aug;35(4):275-278.
The objective of the study was to investigate whether the lysosomal enzyme, N-Acetyl-beta-D-glucosaminidase (NAG) activity is increased in plasma of patients with acute myocardial infarction (AMI) and to determine if...
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Development of an efficient endothelial cell specific vector using promoter and 5' untranslated sequences from the human preproendothelin-1 gene

Cho JY, Lim WC, Jang S, Lee YJ

  • KMID: 1109315
  • Exp Mol Med.
  • 2003 Aug;35(4):269-274.
We report here, that a vector constructed based on ppET-1 gene promoter and 5' untranslated region induced a high level of gene expression in endothelial cells and the specificity is...
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Melatonin reduces ultraviolet-B induced cell damages and polyamine levels in human skin fibroblasts in culture

Lee KS, Lee WS, Suh SI, Kim SP, Lee SR, Ryoo YW, Kim BC

  • KMID: 1109314
  • Exp Mol Med.
  • 2003 Aug;35(4):263-268.
UV radiation is known to cause photoaging of the skin and is considered one of the leading cause of developing skin carcinogenesis. Melatonin which has a highly lipophilic molecular structure...
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Growth promotion of HepG2 hepatoma cells by antisense-mediated knockdown of glypican-3 is independent of insulin-like growth factor 2 signaling

Sung YK, Hwang SY, Farooq M, Kim JC, Kim MK

  • KMID: 1109313
  • Exp Mol Med.
  • 2003 Aug;35(4):257-262.
Glypican-3 (GPC3) encodes a cell-surface heparan-sulfate proteoglycan and its expression is frequently silenced in ovarian cancer, mesotheliomas, and breast cancer cell lines and ectopic expression of GPC3 inhibited the growth...
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Human glutamate dehydrogenase is immunologically distinct from other mammalian orthologues

Jang SH, Kim AY, Bahn JH, Eum WS, Kim DW, Park J, Lee KS, Kang TC, Won MH, Kang JH, Kwon OS, Yoon HY, Lee EY, Cho SW, Choi SY

  • KMID: 1109312
  • Exp Mol Med.
  • 2003 Aug;35(4):249-256.
Five monoclonal antibodies (mAbs) that recognize human glutamate dehydrogenase (GDH) have been selected and designated as monoclonal antibodies hGDH60-6, hGDH60-8, hGDH63-10, hGDH63-11, and hGDH91-14. A total of five mAbs recognizing...
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Study of the difference of high and low metastasis cell line's gene expression map and metastasis-related genes of adenoid cystic carcinoma

Zhu N, Yang J, Wang Y, Guan X

  • KMID: 1109311
  • Exp Mol Med.
  • 2003 Aug;35(4):243-248.
We searched for metastasis-related genes in adenoid cystic carcinoma by suppression subtractive hybridization analysis of high and low metastasis cell lines. Twelve genes (ten previously identified and two novel sequences)...
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A model for the reaction mechanism of the transglutaminase 3 enzyme

Ahvazi B, Steinert P

  • KMID: 1109310
  • Exp Mol Med.
  • 2003 Aug;35(4):228-242.
Transglutaminase enzymes (TGases) catalyze the calcium dependent formation of an isopeptide bond between protein-bound glutamine and lysine substrates. Previously we have shown that activated TGase 3 acquires two additional calcium...
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