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Molecular cloning and sequencing of rat Cdc42 GTPase cDNA

Han JS, Kim JH, Kim JG, Park JB, Noh DY, Lee KH

  • KMID: 755074
  • Exp Mol Med.
  • 2000 Sep;32(3):115-119.
Cdc42 is a member of the Rho family of small GTP-ase and plays an important role in intracellular signaling pathways regulating cell morphology, motility and stimulation of DNA...
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Identification of Escherichia coli 8-oxoguanine endonuclease

Lee YS, Chung MH

  • KMID: 755080
  • Exp Mol Med.
  • 2000 Sep;32(3):155-160.
7,8-Dihydro-8-oxoguanine (oh8Gua) endonuclease is a DNA repair enzyme in Escherichia coli to remove oh8Gua, a promutagenic DNA adduct. Due to the unique mode of enzyme action and substrate specificity, this...
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The mechanism of phospholipase C-gamma1 regulation

Kim MJ, Kim EK, Ryu SH, Suh PG

  • KMID: 755072
  • Exp Mol Med.
  • 2000 Sep;32(3):101-109.
Phospholipase C (PLC)1 hydrolyzes phosphatidylinositol 4,5-bisphosphate to generate the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). IP3 induces a transient increase in intracellular free Ca2+, while...
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Molecular assembly of mitogen-activated protein kinase module in ras-transformed NIH3T3 cell line

Park KS, Kim JA, Chai KJ

  • KMID: 755075
  • Exp Mol Med.
  • 2000 Sep;32(3):120-126.
The ras, is a G-like protein that controls the mitogen-activated protein kinase (MAPK) pathway involved in control and differentiation of cell growth. MAPK is a key component of its...
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An improved method for quantitative sugar analysis of glycoproteins

Kim S, Kim SH, Kim SI, Ha KS, Leem SH

  • KMID: 755078
  • Exp Mol Med.
  • 2000 Sep;32(3):141-145.
Although there are numerous methods available to hydrolyze glycans utilizing strong acids, it all requires lengthy steps to obtain quantitative yield. We have developed a new simple one-step method for...
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Effect of short-term ethanol on the proliferative response of Swiss 3T3 cells to mitogenic growth factors

Yeo EJ, Lim HK, Park SC

  • KMID: 755081
  • Exp Mol Med.
  • 2000 Sep;32(3):161-169.
Both adaptive and deleterious responses of cells to ethanol are likely triggered by short-term interactions of the cells with ethanol. Many studies have demonstrated the direct effect of ethanol...
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Molecular characteristics of the inhibition of human neutrophil elastase by nonsteroidal antiinflammatory drugs

Kang K, Bae SJ, Kim WM, Lee DH, Cho US, Lee MS, Lee MH, Nam SI, Kuettner K, Schwartz D

  • KMID: 755079
  • Exp Mol Med.
  • 2000 Sep;32(3):146-154.
Nonsteroidal antiinflammatory drugs(NSAIDs) are known as clinically effective agents for treatment of inflammatory diseases. Inhibition of cyclooxygenase has been thought to be a major facet of the pharmacological mechanism of...
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Differential changes in the expression of cyclic nucleotide phosphodiesterase isoforms in rat brains by chronic treatment with electroconvulsive shock

Cho CH, Cho DH, Seo MR, Juhnn YS

  • KMID: 755073
  • Exp Mol Med.
  • 2000 Sep;32(3):110-114.
Electroconvulsive shock (ECS) has been suggested to affect cAMP signaling pathways to exert therapeutic effects. ECS was recently reported to increase the expression of PDE4 isoforms in rat brain,...
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Dexamethasone enhances phospholipase D activity in M-1 cells

Kim WJ, Lee MJ, Park MA, Jung JS, Uhlinger D, Kwak JY

  • KMID: 755082
  • Exp Mol Med.
  • 2000 Sep;32(3):170-177.
Phospholipase D (PLD) is an enzyme involved in signal transduction and widely distributed in mammalian cells. The signal transduction pathways and role for phospholipid metabolism during hormonal response in...
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Responsive site on the thrombospondin-1 promotor to down-regulation by phorbol 12-myristate 13-acetate in porcine aortic endothelial cells

Kim SA, Hong KJ

  • KMID: 755077
  • Exp Mol Med.
  • 2000 Sep;32(3):135-140.
Thrombospondin-1 (TSP-1), a multifunctional extracellular matrix protein, inhibits neovascularization and is implicated in the regression of tumor growth and metastasis. We found that the synthesis of TSP-1 in...
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Effects of allyl sulfur compounds and garlic extract on the expression of Bcl-2, Bax, and p53 in non small cell lung cancer cell lines

Hong YS, Ham YA, Choi JH, Kim JG

  • KMID: 755076
  • Exp Mol Med.
  • 2000 Sep;32(3):127-134.
Allyl sulfur compounds play a major role in the chemoprevention against carcinogenesis. The present study compared the antiproliferative effects of diallyl sulfide (DAS), diallyl disulfide (DADS) and garlic...
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