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A molecular model of a point mutation (Val297Met) in the serine protease domain of protein C

Song KS, Park YS, Choi JR, Kim HK, Park Q

  • KMID: 755027
  • Exp Mol Med.
  • 1999 Mar;31(1):47-51.
A heterozygous GTG to ATG (Val297Met) mutation was detected in a patient with inherited protein C deficiency and deep vein thrombosis. Cosegregation of the mutation with protein C deficiency was observed through...
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Co-activation of Gi and Gq proteins exerts synergistic effect on human platelet aggregation through activation of phospholipase C and Ca2+ signalling pathways

Shah BH, Siddiqui A, Qureshi KA, Khan M, Rafi S, Ujan VA, Yakoob MY, Rasheed H, Saeed SA

  • KMID: 755026
  • Exp Mol Med.
  • 1999 Mar;31(1):42-46.
Our previous studies have shown that subthreshold concentrations of two platelet agonists exert synergistic effects on platelet aggregation. Here we studied the mechanism of synergistic interaction of 5-hydroxytryptamine (5-HT) and epinephrine mediated platelet...
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Retroactive DNA analysis for sex determination and dystrophin gene by polymerase chain reaction with archived cytogenetic slides

Choi SK, Kim JW, Park SY, Kim YM, Kim JM, Ryu HM, Yang JS, Yoon SR

  • KMID: 755025
  • Exp Mol Med.
  • 1999 Mar;31(1):36-41.
We describe a rapid and efficient diagnostic method for sex determination and the dystrophin gene by the polymerase chain reaction (PCR) using archived cytogenetic slides. Archived cytogenetic slides stored for about 4...
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Cysteine carboxyl O-methylation of human placental 23 kDa protein

Hong KM, Choi YB, Hong JH, Chang HS, Rhee KI, Park H, Paik MK

  • KMID: 755024
  • Exp Mol Med.
  • 1999 Mar;31(1):30-35.
C-Terminal carboxyl methylation of a human placental 23 kDa protein catalyzed by membrane-associated methyltransferase has been investigated. The 23 kDa protein substrate methylated was partially purified by DEAE-Sephacel, hydroxyapatite and Sephadex G-100 gel...
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Effect of amino acids on inhibition of lactate dehydrogenase-X by gossypol

Javed MH, Khan MA

  • KMID: 755023
  • Exp Mol Med.
  • 1999 Mar;31(1):25-29.
Gossypol acetic acid (GAA) has been shown to have male antifertility effects, but there are pronounced differences among animal species. In the search of endogenous effector molecules, which interfere with the functions...
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Improved cycle sequencing of GC-rich DNA template

Choi JS, Kim JS, Joe CO, Kim SH, Ha KS, Park YM

  • KMID: 755022
  • Exp Mol Med.
  • 1999 Mar;31(1):20-24.
Even when DNA sequencing of purified DNA template failed under the optimal condition, it can be generally contributed to high GC content. GC-rich region of template causes a secondary structure to produce...
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Bricks and mortar of the epidermal barrier

Nemes Z, Steinert P

  • KMID: 755021
  • Exp Mol Med.
  • 1999 Mar;31(1):5-19.
A specialized tissue type, the keratinizing epithelium, protects terrestrial mammals from water loss and noxious physical, chemical and mechanical insults. This barrier between the body and the environment is constantly maintained by reproduction...
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A possible evolutionary role of formaldehyde

Kalapos MP

  • KMID: 755020
  • Exp Mol Med.
  • 1999 Mar;31(1):1-4.
Formaldehyde is a compound which is believed to have had a role in evolutionary processes. On the other hand, the (methyl)glyoxalase pathway is a route being present in all biological organisms whereas...
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