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OBJECTIVE: To find out the optimal conditions of human chromosomal analysis protocol in peripheral blood sample.
METHODS: The experiments were made with the variations of phytohaemagglutinin, colcemid, ethidium bromide concentration and...
BACKGROUND: The combination of Philadelphia chromosome (Ph) and monosomy 7(-7) was rarely observed in acute lymphoblastic leukemia (ALL). With the results from immunophenotyplc and molecular analysis, Philadelphia chromosome positive ALL...
BACKGROUND: The KCl hypotonic treatment is important for swelling the cells and adequate spreading of chromosomes on the slide. Cytogenetic laboratory usually use 0.075M KCl solution. Sometimes, it is difficult...
Lim CK, Min DM, Lee HS, Kim JY, Koong MK, Kang IS, Jun JH
KMID: 1979046
Korean J Fertil Steril.
2003 Jun;30(2):111-118.
OBJECTIVES: The development of an useful method for obtaining metaphase chromosomes from a biopsied blastomere would allow differentiation between embryos with balanced and normal chromosome complements in the preimplantation genetic...