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A radioimmunoassay method for detection of DNA based on chemical immobilization of anti-DNA antibody

Yoo SK, Yoon MO, Park UJ, Han HS, Kim JH, Hwang HJ

  • KMID: 755039
  • Exp Mol Med.
  • 1999 Sep;31(3):122-125.
High selectivity provided by biomolecules such as antibodies and enzymes has been exploited during the last two decades for development of biosensors. Of particular importance are efficient immobilization methods for biomolecules in order...
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Colorectal Cancer Screening: Stool DNA and Other Noninvasive Modalities

Bailey JR, Aggarwal A, Imperiale

Colorectal cancer screening dates to the discovery of pre-cancerous adenomatous tissue. Screening modalities and guidelines directed at prevention and early detection have evolved and resulted in a significant decrease in...
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TIGR gene in primary open-angle glaucoma and steroid-induced glaucoma

Kee C, Ahn BH

To identify TIGR gene mutation in two Korean pedigrees of primary open-angle glaucoma (POAG), and in 25 steroid-induced glaucoma patients, TIGR gene assay was performed. Genomic DNA was extracted from...
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Retroactive DNA analysis for sex determination and dystrophin gene by polymerase chain reaction with archived cytogenetic slides

Choi SK, Kim JW, Park SY, Kim YM, Kim JM, Ryu HM, Yang JS, Yoon SR

  • KMID: 755025
  • Exp Mol Med.
  • 1999 Mar;31(1):36-41.
We describe a rapid and efficient diagnostic method for sex determination and the dystrophin gene by the polymerase chain reaction (PCR) using archived cytogenetic slides. Archived cytogenetic slides stored for about 4...
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Comparison of the reverse transcription-PCR with the branched DNA assay for measurement of human immunodeficiency virus type 1 RNA levels in plasma of Korean patients

Won DI, Park JY, Kim JM, Kim HS

Viral load testing of human immunodeficiency virus (HIV) is an essential tool for initiating and monitoring the antiretroviral therapy for HIV patients. To this end, several methods...
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Cowden Syndrome Presenting as Breast Cancer: Imaging and Clinical Features

Seo M, Cho N, Ahn HS, Moon HG

Cowden syndrome is an uncommon, autosomal dominant disease which is characterized by multiple hamartomas of the skin, mucous membrane, brain, breast, thyroid, and gastrointestinal tract. The diagnosis of Cowden syndrome...
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Molecular analysis of prion protein gene (PRNP) in Korean patients with Creutzfeldt-Jakob disease

Jeong BH, Ju WK, Huh K, Lee EA, Choi IS, Im JH, Choi EK, Kim YS

Creutzfeldt-Jakob disease (CJD), a relatively uncommon human dementia, is caused by an unconventional slow infectious agent. Several cases of CJD, clinically or histopathologically diagnosed, have been reported in Korea....
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TGF-beta1 inhibition of apoptosis through the transcriptional up-regulation of Bcl-X(L) in human monocytic leukemia U937 cells

Lee JH, Park BJ, Park JH, Yang MH, Chi SG

  • KMID: 755040
  • Exp Mol Med.
  • 1999 Sep;31(3):126-133.
To characterize the TGF-beta1 response of monocytic leukemia cells, we analyzed the effects of TGF-beta1 on cell proliferation, differentiation, and apoptosis of human monoblastic U937 cells. Treatment of cells with TGF-beta1 in...
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Frequency of FCGR3B Alleles in Thai Blood Donors

Kaset C, Leetrakool N, Intharanut K, Nathalang

BACKGROUND: Human neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described...
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Individual Variation in Growth Factor Concentrations in Platelet-rich Plasma and Its Influence on Human Mesenchymal Stem Cells

Cho HS, Song IH, Park SY, Sung MC, Ahn MW, Song KE

BACKGROUND: The objective of this study was to explore whether individual variations in the concentration of growth factors (GFs) influence the biologic effects of platelet-rich plasma (PRP) on human mesenchymal...
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Detection of cryptic Y chromosome mosaicism by coamplification PCR with archived cytogenetic slides of suspected Turner syndrome

Kim JW, Cho EH, Kim YM, Kim JM, Han JY, Park SY

  • KMID: 927132
  • Exp Mol Med.
  • 2000 Mar;32(1):38-41.
Turner syndrome is one of the most common cytogenetic abnormalities. It is known that the Y chromosome or Y derived material is present in 6-9% of TS patient and it...
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Cell cycling status of human cord blood CD34+ cells during ex vivo expansion is related to the level of very late antigen expression

Seoh JY, Park HY, Chung WS, Kim SC, Hahn MJ, Kim KH, Shin HY, Ahn HS, Park KW, Ryu KH

Very late antigen-4 (VLA-4), which binds to the extracellular matrix protein fibronectin, is an integrin molecule known to be modulated during mobilization of CD34+ cells, and to be involved in...
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Differentiation of Korean isolates of Entamoeba histolytica from Entamoeba dispar

Choe SC, Lee M, Lee SK, Im K, Tannich E, Lee SH, Hong ST

Cysts of Entamoeba histolytica are still found from humans in Korea, but not all of the cysts are known as pathogenic. The non-pathogenic strain is regarded as a different species,...
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Potential Risk Factors Associated With Vascular Diseases in Patients Receiving Treatment for Hypertension

Kim H, Park J, Chae H, Lee GD, Lee SY, Lee JM, Oh YS, Kim M, Kim Y

BACKGROUND: Currently, the hypertension (HTN) patients undergo appropriate medical treatment, and traditional risk factors are highly controlled. Therefore, potential risk factors of atherosclerotic vascular diseases (AVD) and venous thromboembolisms (VTE)...
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