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In Vitro Developments of Porcine Embryos Produced by In Vitro Fertilization and Parthenogenetic Activation

Uhm SJ, Kim NH, Kim EY, Kim MK, Cha SK, Yoon SH, Park SP, Chung KS, Lim JH

  • KMID: 1930555
  • Korean J Fertil Steril.
  • 1997 Apr;24(1):43-49.
The objective of this study is to determine developmental pattern and cell allocation to the ICM and TE in haploid and diploid of embryos following parthenogenetic activation and in vitro...
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The Effects of Cytochalasin B and Colchicine on Fine Structure of Bile Canaliculi and Hepatocytes in Mouse Liver

Park CH, Jang BJ, Uhm CS

  • KMID: 1530060
  • Korean J Anat.
  • 1998 Apr;31(2):241-251.
Bile canaliculi is closely related to the cytoskeleton; actin filament web, microtubules and cytokeratin intermediate filaments. To understand how cytoskeletal alteration affects bile canalicular structure, the investigators injected cytochalasin B...
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Hepatocyte Lacks Insulin-Mediated Glucose Transporter Translocation Mechanism in Rat

Hah JS

The subcellular distribution of glucose transporter in rat hepatocytes was studied in the absence and presence of insulin by measuring glucose-sensitive cytochalasin B binding sites and immunoreactivities to antibodies specific...
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Cryopreservation of Testicular Spermatozoa using Mouse Zona Pellucida in Intracytoplasmic Sperm Injection Program

Suh TK, Jeon BG, Ryu EK, Lee ES, Ryoo ZY, Sohn SH, Moon JS, Kim KC

  • KMID: 1531504
  • Korean J Fertil Steril.
  • 1997 Aug;24(2):187-192.
The survival rate and motility recovered after cryopreservation of testicular spermatozoa in testicular sperm extraction (TESE)-ICSl program is low. The purpose of this study was to assess the availability and...
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The Effects of Physical States of Phospholipids on the Incorporation and CB Binding Activity of Band 4.5 Protein of human Erythrocyte Membrane in Reconstituted Vesicles

Hah JS

Proteoliposomes were prepared by reconstitution of a solubilized band 4.5 protein of human erythrocyte membrane with a mixture of phosphatidylcholine(PC) and phosphatidylethanolamine(PE) of varying ratios. The incorporation of the band...
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Effect of Cytochalasin B on Survivability and in Vitro Development of Mouse Oocyte Frozen by Vitrification

Choi WJ, Lee SA, Lee JH, Paik WY

  • KMID: 2271962
  • Korean J Obstet Gynecol.
  • 2003 Feb;46(2):317-322.
OBJECTIVE: The purpose of this study was to evaluate the effect of CCB treatment on the survivability and in vitro development of mouse oocyte frozen by vitrification. METHODS: Mouse oocytes retrieved...
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The Effect of Vanadate on Glucase Transport of Everted Sacs and Vesicles Prepared from Small Intestinal Epithelial Cells

Hahm JS, Kim KJ

Vanadate has been reported to inhibit Na+, K+-ATPase of many cells. Since intestinal uptake of glucose is influenced by the enzyme, we studied the effects of varying concentrations of sodium...
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Cytochalasin B Modulates Macrophage-Mediated Inflammatory Responses

Kim MY, Kim JH, Cho JY

  • KMID: 2170622
  • Biomol Ther.
  • 2014 Jul;22(4):295-300.
The actin cytoskeleton plays an important role in macrophage-mediated inflammatory responses by modulating the activation of Src and subsequently inducing nuclear factor (NF)-kappaB translocation. In spite of its critical functions,...
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The Role of Vibrio vulnificus Capsular Polysaccharide as a Virulence Factor

Chung SS, Lee HC, Shin BA, Lee SE, Ryu PY, Kim WS

  • KMID: 2099291
  • J Korean Soc Microbiol.
  • 1998 Oct;33(5):425-434.
To evaluate the role of capsular polysaccharide (CPS) as a virulence factor, the interaction of V. vulnificus with mouse peritoneal macrophages and serum, which are involved in the clearance of...
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A study on the regulation of translocation of glucose transporters during hepatocarcinogenesis induced by 3'-Me DAB

Kim YS, Paik EM, Lee MS, Kim KS, Chai KJ, Ahn YH

The mechanism of glucose transported (GT) expression on the plasma membranes of hepatoma cells in rats induced by 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB) was studied. Cytochalasin B binding to plasma membrane fractions from...
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Effects of Bisphosphonates on Glucose Transport in a Conditionally Immortalized Rat Retinal Capillary Endothelial Cell Line (TR-iBRB Cells)

Lee NY, Park HJ, Kang YS

The objective of the present study was to elucidate the effect of bisphosphonates, anti-osteoporosis agents, on glucose uptake in retinal capillary endothelial cells under normal and high glucose conditions. The...
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Swiprosin-1 Regulates Cytokine Expression of Human Mast Cell Line HMC-1 through Actin Remodeling

Ramesh TP, Kim YD, Kwon MS, Jun CD, Kim SW

BACKGROUND: Swiprosin-1 was identified in human CD8+ lymphocytes, mature B cells and non-lymphonoid tissue. We have recently reported that swiprosin-1 is expressed in mast cells and up-regulated in both in...
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Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

Manjunatha BM, Ravindra J, Gupta PS, Devaraj M, Honnappa T, Krishnaswamy A

The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium...
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Appropriate In Vitro Methods for Genotoxicity Testing of Silver Nanoparticles

Kim HR, Park YJ, Shin DY, Oh SM, Chung KH

  • KMID: 2380115
  • Environ Health Toxicol.
  • 2013 ;28(1):e2013003.
OBJECTIVES: We investigated the genotoxic effects of 40-59 nm silver nanoparticles (Ag-NPs) by bacterial reverse mutation assay (Ames test), in vitro comet assay and micronucleus (MN) assay. In particular, we...
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Protein tyrosine phosphatase 1B is a mediator of cyclic ADP ribose-induced Ca²⁺ signaling in ventricular myocytes

Park SA, Hong BZ, Ha KC, Kim UH, Han MK, Kwak YG

Cyclic ADP-ribose (cADPR) releases Ca²⁺ from ryanodine receptor (RyR)-sensitive calcium pools in various cell types. In cardiac myocytes, the physiological levels of cADPR transiently increase the amplitude and frequency of...
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Effects of glutamate on dehydroascorbate uptake and Its enhanced vulnerability to the peroxidation in cerebral cortical slices

Song JH, Shin SH, Chung IM

  • KMID: 1121095
  • Exp Mol Med.
  • 2002 Dec;34(6):419-425.
Pro-oxidant properties of ascorbate have been studied with uses of brain tissues and neuronal cells. Here we address potential mechanism of ascorbate coupling with glutamate to generate oxidative stress,...
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