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Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System

Lim KT, Lee BC, Kang SK, Hwang WS

  • KMID: 1099824
  • J Vet Sci.
  • 2003 Apr;4(1):73-78.
In this study, we examined the effects of a two-step culture system, which involves the use of different culture media for early cleavage and later stage embryos, on the in...
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Human embryonic stem cells and therapeutic cloning

Hwang WS, Lee BC, Lee CK, Kang SK

  • KMID: 1114848
  • J Vet Sci.
  • 2005 Jun;6(2):87-96.
The remarkable potential of embryonic stem (ES) cells is their ability to develop into many different cell types. ES cells make it possible to treat patients by transplanting specialized healthy...
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Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Cho ES, Lee SY, Park JY, Hong SG, Ryu PD

Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged...
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Elevated Contractile Responses to Acetylcholine in Organ Cultured Rabbit Carotid Artery

Lee Y, Jung S, Won JE, Nam T, Ahn D

The aim of the present study was to examine the functional changes that occur when a rabbit carotid artery is cultured in serum-free medium. In endothelium (EC)-intact arteries cultured under...
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A new culture system for in situ observation of the growth and development of Eucyclops serrulatus (Copepoda: Cyclopoida)

Park SH, Chang CY, Shin SS

A practical and convenient method of rearing Eucyclops serrulatus in a microculture environment is described. A complete life cycle of E. serrulatus was maintained in a narrow space on a...
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Efficient culture system for human embryonic stem cells using autologous human embryonic stem cell-derived feeder cells

Yoo SJ, Yoon BS, Kim JM, Song JM, Roh SI, You S, Yoon HS

  • KMID: 1089449
  • Exp Mol Med.
  • 2005 Oct;37(5):399-407.
Human embryonic stem cells (hESCs) need feeder cells for their maintenance in an undifferentiated state. In conventional culture systems, mouse embryonic fibroblasts (MEFs) serve as feeder cells to maintain hESCs....
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Platelet-rich plasma inhibits the apoptosis of highly adipogenic homogeneous preadipocytes in an in vitro culture system

Fukaya Y, Kuroda M, Aoyagi Y, Asada S, Kubota Y, Okamoto Y, Nakayama T, Saito Y, Satoh K, Bujo H

  • KMID: 1383794
  • Exp Mol Med.
  • 2012 May;44(5):330-339.
Auto-transplantation of adipose tissue is commonly used for the treatment of tissue defects in plastic surgery. The survival of the transplanted adipose tissue is not always constant, and one of...
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Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

Gao J, Meng C, Chen Z, Li C, Liu G

Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit...
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Tissue engineering of heart valves by recellularization of glutaraldehyde-fixed porcine valves using bone marrow-derived cells

Kim SS, Lim SH, Cho SW, Gwak SJ, Hong YS, Chang BC, Park MH, Song KW, Choi CY, Kim BS

  • KMID: 1094153
  • Exp Mol Med.
  • 2006 Jun;38(3):273-283.
To increase the biocompatibility and durability of glutaraldehyde (GA)-fixed valves, a biological coating with viable endothelial cells (ECs) has been proposed. However, stable EC layers have not been formed successfully...
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