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Human platelet lysate efficiency, stability, and optimal heparin concentration required in culture of mammalian cells

Mohamed HE, Asker ME, Kotb NS, El Habab A

BACKGROUND: Fetal bovine serum (FBS) has been used to support the growth and proliferation of mammalian cells for decades. Owing to several risk factors associated with FBS, several trials have...
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Establishment of patient-derived three-dimensional organoid culture in renal cell carcinoma

Na JC, Kim JH, Kim SY, Gu YR, Jun DY, Lee HH, Yoon YE, Choi KH, Hong SJ, Han WK

PURPOSE: Renal cell carcinoma is a heterogeneous kidney cancer, and over 403,000 cases were reported worldwide in 2018. Current methods for studying renal cell carcinoma are limited to two-dimensional (2D)...
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Comparison of antigenic mutation during egg and cell passage cultivation of H3N2 influenza virus

Park YW, Kim YH, Jung HU, Jeong OS, Hong EJ, Kim H, Lee JI

PURPOSE: When influenza viruses are cultured in eggs, amino acid mutations of the hemagglutinin may occur through egg adaptation. On the other hand, when influenza viruses are cultured in animal...
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Suspension culture of Vero cells for the production of adenovirus type 5

Lee DK, Park J, Seo DW

PURPOSE: Most cell culture processes for viral vaccine production are mainly based on adherent cell culture systems using serum, which are associated with expensive and labor-intensive processes to produce large...
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Regulation of porcine endogenous retrovirus by dual LTR1+2 (Long Terminal Region) miRNA in primary porcine kidney cells

Chung HC, Nguyen VG, Moon HJ, Park YH, Park BK

Porcine endogenous retroviruses (PERVs) integrate into germline DNA as proviral genome that enables vertical transmission from parents to their offspring. The provirus usually survives as part of the host genome...
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Decursinol Angelate Ameliorates Dextran Sodium Sulfate-Induced Colitis by Modulating Type 17 Helper T Cell Responses

Thapa B, Pak S, Kwon HJ, Lee K

Angelica gigas has been used as a Korean traditional medicine for pain relief and gynecological health. Although the extracts are reported to have an anti-inflammatory property, the bioactive compounds of...
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Applications of photobiomodulation in hearing research: from bench to clinic

Lee JH, Kim S, Jung JY, Lee MY

Hearing loss is very common and economically burdensome. No accepted therapeutic modality for sensorineural hearing loss is yet available; most clinicians emphasize rehabilitation, placing hearing aids and cochlear implants. Photobiomodulation...
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Udenafil Induces the Hair Growth Effect of Adipose-Derived Stem Cells

Choi N, Sung JH

Udenafil, which is a PDE5 inhibitor, is used to treat erectile dysfunction. However, it is unclear whether udenafil induces hair growth via the stimulation of adipose-derived stem cells (ASCs). In...
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Stem cell-based therapy for inflammatory bowel disease

Shimizu H, Suzuki K, Watanabe M, Okamoto R

Inflammatory bowel disease (IBD) is an idiopathic, multi-etiological disease characterized by inflammation and mucosal destruction of the gastrointestinal tract. Despite the remarkable advance in immunomodulating therapies, there still remains a...
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Role of DNA Methylation in the Development and Differentiation of Intestinal Epithelial Cells and Smooth Muscle Cells

Jorgensen , Ro S

  • KMID: 2454636
  • J Neurogastroenterol Motil.
  • 2019 Jul;25(3):377-386.
  • doi: 10.5056/jnm19077
The mammalian intestine contains many different cell types but is comprised of 2 main cell types: epithelial cells and smooth muscle cells. Recent in vivo and in vitro evidence has...
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Zaluzanin C Inhibits Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes

Kwak SH, Kim YH

BACKGROUND: An excess storage of body fat causes obesity. Since obesity increases risk of chronic diseases, it is important to inhibit excessive storage of fat. Zaluzanin C is a sesquiterpene...
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Decontamination methods to restore the biocompatibility of contaminated titanium surfaces

Jin SH, Lee EM, Park JB, Kim KK, Ko Y

PURPOSE: The reaction of cells to a titanium implant depends on the surface characteristics of the implant which are affected by decontamination. The aim of this study was to evaluate...
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Helicobacter pylori inhibited cell proliferation in human periodontal ligament fibroblasts through the Cdc25C/CDK1/cyclinB1 signaling cascade

Li H, Liang D, Hu N, Dai X, He J, Zhuang H, Zhao W

PURPOSE: Several studies have shown that the oral cavity is a secondary location for Helicobacter pylori colonization and that H. pylori is associated with the severity of periodontitis. This study...
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Cytopathic Change and Inflammatory Response of Human Corneal Epithelial Cells Induced by Acanthamoeba castellanii Trophozoites and Cysts

Sohn HJ, Seo GE, Lee JH, Ham AJ, Oh YH, Kang H, Shin HJ

Acanthamoeba castellanii has ubiquitous distribution and causes primary acanthamoebic keratitis (AK). AK is a common disease in contact lens wearers and results in permanent visual impairment or blindness. In this...
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Epidural Fat-Derived Mesenchymal Stem Cell: First Report of Epidural Fat-Derived Mesenchymal Stem Cell

Lee GW, Seo MS, Kang KK, Oh SK

STUDY DESIGN: Experimental study. PURPOSE: To determine whether epidural fat (EF) tissue contains mesenchymal stem cells (MSC). OVERVIEW OF LITERATURE: Spine surgeons are unaware of the contents of EF tissue and the...
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Metformin, an Activator of AMPK, Promotes the Growth of Hair Follicles via the AMPK/β-catenin Signaling Pathway

Moon HR, Park KY, Kang HJ, Lee WJ, Won CH, Chang SE, Lee MW, Choi JH, Park GH

  • KMID: 2449198
  • Korean J Dermatol.
  • 2019 May;57(4):175-181.
BACKGROUND: AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase that plays a pivotal role in the balance of cellular energy metabolism. Recent studies have reported that AMPK has numerous...
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Antiallergic Effect of Hizikia fusiformis in an Ovalbumin-Induced Allergic Rhinitis Mouse Model

Zhang YL, Shin HJ, Lee JH, Lee J

OBJECTIVES: The extract of Hizikia fusiformis is known to exhibit anticancer, antiatopic and antioxidant activities. We aimed to investigate the extract of H. fusiformis on allergic rhinitis inflammation in a...
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D-RADA16-RGD-Reinforced Nano-Hydroxyapatite/Polyamide 66 Ternary Biomaterial for Bone Formation

Zhao W, He B, Zhou A, Li Y, Chen X, Yang Q, Chen B, Qiao B, Jiang D

BACKGROUND: Nano-hydroxyapatite/polyamide 66 (nHA/PA66) is a composite used widely in the repair of bone defects. However, this material is insufficient bioactivity. In contrast, D-RADA16-RGD self-assembling peptide (D-RADA16-RGD sequence containing all...
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RNA-sequencing Profiles of Cell Cycle–Related Genes Upregulated during the G2-Phase in Giardia lamblia

Kim J, Shin MY, Park SJ

To identify the component(s) involved in cell cycle control in the protozoan Giardia lamblia, cells arrested at the G1/S- or G2-phase by treatment with nocodazole and aphidicolin were prepared from...
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Bioreactor systems are essentially required for stem cell bioprocessing

Cha JM, Lee MY, Hong J

As stem cell technologies have rapidly advanced, the stem cell therapy market has been forecast to reach hundreds of millions of USD in market value within the next 5 years....
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