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Diagnosis of bovine cryptosporidiosis by indirect immunofluorescence assay using monoclonal antibody

Wee SH, Lee CG, Joo HD

Two hybridoma cell lines against Cryptosporidium parvum oocysts (VRI- CN91) were produced. The isotype of these 2 monoclonal antibodies (mAbs) was IgG2b (1E7.2) and and IgM (C6). Enzyme immuno-transfer blotting...
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Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP

Cheun HI, Kim K, Yoon S, Lee WJ, Park WY, Sim S, Yu JR

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between...
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Internal Amplification Control for a Cryptosporidium Diagnostic PCR: Construction and Clinical Evaluation

Hawash Y, Ghonaim MM, Al-Hazmi A

Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it...
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