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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoeba sp. YM-4 (Korean isolate)

Shin HJ, Im K, Jeon KW

Acanthamoeba sp. YM-4 is similar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in vitro cytotoxicity and isoenzyme patterns....
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Molecular characterization of bacterial endosymbionts of Acanthamoeba isolates from infected corneas of Korean patients

Xuan YH, Yu HS, Jeong HJ, Seol SY, Chung DI, Kong HH

The endosymbionts of 4 strains of Acanthamoeba (KA/E9, KA/E21, KA/E22, and KA/E23) isolated from the infected corneas of Korean patients were characterized via orcein stain, transmission electron microscopic examination, and...
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene

Yu HS, Hwang MY, Kim TO, Kim TH, Kong HH, Chung DI, Park JW

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships. Twenty-five isolates representing 20 species were...
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Natural occurrence of Mycobacterium as an endosymbiont of Acanthamoeba isolated from a contact lens storage case

Yu HS, Jeong HJ, Hong YC, Seol SY, Chung DI, Kong HH

Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within freeliving amoebae. It is believed that protozoans function as host cells for the intracellular replication...
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Molecular and biochemical characterization of a novel actin bundling protein in Acanthamoeba

Alafag JI, Moon EK, Hong YC, Chung DI, Kong HH

Actin binding proteins play key roles in cell structure and movement particularly as regulators of the assembly, stability and localization of actin filaments in the cytoplasm. In the present study,...
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Upregulated expression of the cDNA fragment possibly related to the virulence of Acanthamoeba culbertsoni

Im KI, Park KM, Yong TS, Hong YP, Kim TE

Identification of the genes responsible for the recovery of virulence in brain-passaged Acanthamoeba culbertsoni was attempted via mRNA differential display-polymerase chain reaction (mRNA DD-PCR) analysis. In order to identify...
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