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Restriction endonuclease analysis of mitochondrial DNA of Acanthamoeba sp. YM-4 (Korean isolate)

Shin HJ, Im K, Jeon KW

Acanthamoeba sp. YM-4 is similar to A. culbertsoni based upon morphological characteristics of trophozoites and cysts. However, based on other characteristics, pathogenicity to mice, in vitro cytotoxicity and isoenzyme patterns....
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Pathogenic free-living amoebae in Korea

Shin HJ, Im KI

Acanthamoeba and Naegleria are widely distributed in fresh water, soil and dust throughout the world, and cause meningoencephalitis or keratoconjunctivitis in humans and other mammals. Korean isolates, namely, Naegleria sp....
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A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level

Kong HH, Chung DI

We describe a riboprinting scheme for identification of unknown Acanthamoeba isolates at the species level. It involves use of the PCR-RFLP of small subunit ribosomal RNA gene (riboprint) of...
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Comparison of virulence by Acanthamoeba strains in a murine model of acquired immunodeficiency syndrome

Kong HH, Yee ST, Chung DI

The pathogenic potential of Acanthamoeba strains was evaluated by experimental infection of murine AIDS (MAIDS) model. C57BL/6 mice were induced to immunocompromized state by intraperitoneal injection of LP-BM5 MuLV and...
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Keratitis by Acanthamoeba triangularis: Report of Cases and Characterization of Isolates

Xuan YH, Chung BS, Hong YC, Kong HH, Hahn TW, Chung DI

Three Acanthamoeba isolates (KA/E9, KA/E17, and KA/E23) from patients with keratitis were identified as Acanthamoeba triangularis by analysis of their molecular characteristics, a species not previously recognized to be a...
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Species identification and molecular characterization of Acanthamoeba isolated from contact lens paraphernalia

Lee SM, Choi YJ, Ryu HW, Kong HH, Chung DI

We applied ribosomal RNA gene (rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify Acanthamoeba isolates from contact lens paraphernalia, and characterized these on the basis of mitochondrial DNA...
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Phylogenetic relationships among Acanthamoeba spp. based on PCR-RFLP analyses of mitochondrial small subunit rRNA gene

Yu HS, Hwang MY, Kim TO, Kim TH, Kong HH, Chung DI, Park JW

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships. Twenty-five isolates representing 20 species were...
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Comparison of specific activity and cytopathic effects of purified 33 kDa serine proteinase from Acanthamoeba strains with different degree of virulence

Kim WT, Kong HH, Ha YR, Hong YC, Jeong HJ, Yu HS, Chung DI

The pathogenic mechanism of granulomatous amebic encephalitis (GAE) and amebic keratitis (AK) by Acanthamoeba has yet to be clarified. Protease has been recognized to play an important role in the...
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Genetic Characterization of Clinical Acanthamoeba Isolates from Japan using Nuclear and Mitochondrial Small Subunit Ribosomal RNA

Rahman MM, Yagita K, Kobayashi , Oikawa Y, Hussein AI, Matsumura , Tokoro M

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by...
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Molecular characterization of Acanthamoeba isolated from amebic keratitis related to orthokeratology lens overnight wear

Lee SJ, Jeong HJ, Lee JE, Lee JS, Xuan YH, Kong HH, Chung DI, Ock MS, Yu HS

In an effort to characterize, on the molecular scale, the Acanthamoeba initially isolated from the cornea of an amoebic keratitis patient associated with overnight-wear orthokeratology lens in Korea, we conducted...
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Genetic analyses of Acanthamoeba isolates from contact lens storage cases of students in Seoul, Korea

Yu HS, Choi KH, Kim HK, Kong HH, Chung DI

We conducted both the small subunit ribosomal DNA (SSU rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and mitochondrial (mt) DNA RFLP analyses for a genetic characterization of Acanthamoeba isolates...
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