Tissue Eng Regen Med.
2022 Apr;19(2):377-387. 10.1007/s13770-021-00423-3.
Tissue Engineering with Compact Bone-Derived Cell Spheroids Enables Bone Formation around Transplanted Tooth
- Affiliations
-
- 1Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University, Shiojiri 399-0781, Japan
- 2Department of Oral and Maxillofacial Surgery, School of Dentistry, Matsumoto Dental University, Shiojiri 399-0781, Japan
- 3Division of Hard Tissue Research, Institute of Oral Science, Matsumoto Dental University, 1780 Hirooka Gobara, Shiojiri 399-0781, Japan
- 4Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai 200031, China
- 5Department of Stomatology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
- 6Department of General Medicine, IMSUT Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo 108-9639, Japan
- KMID: 2528131
- DOI: http://doi.org/10.1007/s13770-021-00423-3
Abstract
- BACKGROUND
Although tooth transplantation is a desirable treatment option for congenital defects of permanent teeth in children, transplantation to a narrow alveolar ridge is not feasible. In this study, we investigated the possibility of bone tissue engineering simultaneously with tooth transplantation to enhance the width of the alveolar bone.
METHODS
Bone marrow mononuclear cells or cortical bone-derived mesenchymal stromal cell spheroids were seeded onto atelocollagen sponge and transplanted with freshly extracted molars from mice of the same strain. New bone formation around the tooth root was evaluated using micro-computed tomography and histological analysis. Tooth alone, or tooth with scaffold but without cells, was also transplanted and served as controls.
RESULTS
Micro-computed tomography showed new bone formation in the furcation area in all four groups. Remarkable bone formation outside the root was also observed in the cortical bone-derived mesenchymal stromal cell group, but was scarce in the other three groups. Histological analysis revealed that the space between the new bone and the root was filled with collagen fibers in all four groups, indicating that the periodontal ligament was maintained.
CONCLUSION
This study demonstrates the potential of simultaneous alveolar bone expansion employing bone tissue engineering approach using cortical bone-derived mesenchymal stromal cell spheroids for tooth transplantation. The use of an orthotopic transplantation model may further clarify the feasibility and functional recovery of the transplanted tooth over a longer period.
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