Tissue Eng Regen Med.  2022 Feb;19(1):59-72. 10.1007/s13770-021-00393-6.

Preparation and In Vitro Characterization of Gelatin Methacrylate for Corneal Tissue Engineering

Affiliations
  • 1MicroNano System Research Center, College of Information and Computer & Key Lab of Advanced Transducers and Intelligent Control System, Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China
  • 2College of Information Science and Engineering, Hebei North University, Zhangjiakou 075000, China
  • 3College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
  • 4Department of Lacrimal Duct Diseases, Shanxi Eye Hospital, Taiyuan 030002, China

Abstract

BACKGROUND
Corneal disease is second only to cataract considered as the leading cause of blindness in the world, with high morbidity. Construction of corneal substitutes In Vitro by tissue engineering technology to achieve corneal regeneration has become a research hotspot in recent years. We conducted in-depth research on the biocompatibility, physicochemical and mechanical properties of rat bone marrow mesenchymal stem cells (rBM-MSCs)-seeded gelatin methacrylate (GelMA) as a bioengineered cornea.
METHODS
Four kinds of GelMA with different concentrations (7, 10, 15 and 30%) were prepared, and their physicchemical, optical properties, and biocompatibility with rBM-MSCs were characterized. MTT, live/dead staining, cell morphology, immunofluorescence staining and gene expression of keratocyte markers were performed.
RESULTS
7%GelMA hydrogel had higher equilibrium water content and porosity, better optical properties and hydrophilicity. In addition, it is more beneficial to the growth and proliferation of rBM-MSCs. However, the 30%GelMA hydrogel had the best mechanical properties, and could be more conducive to promote the differentiation of rBM-MSCs into keratocyte-like cells.
CONCLUSION
As a natural biological scaffold, GelMA hydrogel has good biocompatibility. And it has the ability to promote the differentiation of rBM-MSCs into keratocyte-like cells, which laid a theoretical and experimental foundation for further tissue-engineered corneal stromal transplantation, and provided a new idea for the source of seeded cells in corneal tissue engineering.

Keyword

Bioengineered cornea; Bone marrow mesenchymal stem cells; Corneal tissue engineering; Gelatin methacrylate; Hydrogel
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