Mycobiology.  2004 Mar;32(1):6-10.

Molecular Marker Related to Fruitbody Color of Flammulina velutipes

Affiliations
  • 1Applied Microbiology Division, National Institute of Agricultural Science & Technology, RDA, Suwon 441-707, Korea. wskong@rda.go.kr
  • 2Department of Biotechnology, Yonam College of Agriculture, Sunghwan-Eup, Chonan 330-802, Korea.
  • 3Department of Agronomy, Konkuk University, Seoul 143-701, Korea.

Abstract

White and brown strains of Flammulina velutipes were inter-crossed. All F1 showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

Keyword

Backcross; Bulked segregant analysis; Flammulina velutipes; Molecular marker

MeSH Terms

Clone Cells
Flammulina*
Genes, vif
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