Mycobiology.  2002 Mar;30(1):1-4.

Molecular Analysis of Exophiala Species Using Molecular Markers

Affiliations
  • 1Division of Biological Sciences, Medical School, Konyang University, Nonsan, Chungnam 320-711, Korea.

Abstract

Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP (restriction fragment length polymorphism) of ribosomal DNA, M-13, (GTG)5, and nucleotide sequences of ribosomal ITS (internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and (GTG)5 markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.

Keyword

Exophiala; Genetic relatedness; (GTG)5; M-13; PCR-RFLP; Ribosomal DNA

MeSH Terms

Base Sequence
DNA, Ribosomal
Exophiala*
Genetic Markers
Nucleotides
Polymorphism, Restriction Fragment Length
DNA, Ribosomal
Genetic Markers
Nucleotides
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