J Breast Cancer.  2013 Jun;16(2):152-158. 10.4048/jbc.2013.16.2.152.

Associations between the Expression of Mucins (MUC1, MUC2, MUC5AC, and MUC6) and Clinicopathologic Parameters of Human Breast Ductal Carcinomas

Affiliations
  • 1Department of Pathology, Breast and Thyroid Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. jhpath.sohn@samsung.com
  • 2Department of Surgery, Breast and Thyroid Cancer Center, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.

Abstract

PURPOSE
Mucins are members of the glycoprotein family expressed in benign and malignant epithelial cells. The aim of this study is to evaluate the relationships between the expression of mucins in breast ductal carcinoma and clinicopathologic parameters.
METHODS
We constructed tumor microarrays based on 240 cases of invasive ductal carcinoma and 40 cases of ductal carcinoma in situ (DCIS) using formalin fixed, paraffin embedded tissues. We examined the expressions of MUC1, MUC2, MUC5AC, and MUC6 by immunohistochemistry.
RESULTS
MUC1 demonstrated cytoplasmic, membranous, apical, and combinative expressions. Other mucins demonstrated cytoplasmic expression. In invasive ductal carcinoma, MUC1, MUC2, MUC5AC, and MUC6 were expressed in 93.6%, 6.2%, 4.8%, and 12.4% of cases, respectively; these rates were slightly, but not significantly, higher than observed in cases of DCIS. MUC1 expression was associated with estrogen receptor (ER) expression and negative MUC1 expression was associated with triple negativity. MUC6 expression was correlated with higher histologic grade, lymphatic invasion, lymph node metastasis, and HER2 positivity. No associations with any other clinicopathologic parameters were observed.
CONCLUSION
Most invasive ductal carcinomas of the breast express MUC1, and this expression is associated with ER expression. MUC6 expression is correlated with some clinicopathologic parameters that are indicators of poor prognosis. To evaluate the role of MUC6 as a potential biomarker, further studies are warranted.

Keyword

Breast neoplasms; Human MUC1 protein; Human MUC6 protein; Mucins

MeSH Terms

Breast
Breast Neoplasms
Carcinoma, Ductal
Carcinoma, Intraductal, Noninfiltrating
Cytoplasm
Epithelial Cells
Estrogens
Formaldehyde
Glycoproteins
Humans
Lymph Nodes
Mucins
Neoplasm Metastasis
Paraffin
Prognosis
Estrogens
Formaldehyde
Glycoproteins
Mucins
Paraffin

Figure

  • Figure 1 MUC1 expression with cytoplasmic, membranous and apical or combinative location. MUC1 expression shows variable subcellular locations, such as cytoplasmic (A), membranous (B), apical (C), and combinative cytoplasmic and membranous (D) (immunohistochemical staining, ×200).

  • Figure 2 MUC2, MUC5AC, and MUC6 expression. MUC2 (A), MUC5AC (B), and MUC6 (C) show cytoplasm expression (immunohistochemical staining, ×200).

  • Figure 3 Kaplan-Meier survival analysis results for MUC1 (A), MUC2 (B), MUC5AC (C), and MUC6 (D). Statistical significance for survival is not observed in any of the mucins.


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