Korean J Otorhinolaryngol-Head Neck Surg.
2009 Jun;52(6):486-491. 10.3342/kjorl-hns.2009.52.6.486.
Effect of Platelet Rich Plasma on Facial Nerve Regeneration in Acute Nerve Injury Model
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Gwangju, Korea. victocho@hanmail.net
- 2Department of Physiology, Chonnam National University Medical School, Gwangju, Korea.
- 3Department of Rehabilitation Medicine, Chonnam National University Medical School, Gwangju, Korea.
- 4Department of Pathology, Chonnam National University Medical School, Gwangju, Korea.
- KMID: 2277063
- DOI: http://doi.org/10.3342/kjorl-hns.2009.52.6.486
Abstract
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BACKGROUND AND OBJECTIVES: The object of this study was to evaluate the effect of platelet rich plasma (PRP) on facial nerve regeneration from an axotomy injury in the guinea pig model.
MATERIALS AND METHOD: Experiments involved the transection and repair of right facial nerve. The right facial nerve of 14 albino guinea pigs were completely transected and immediately sutured, followed by fibrin glue only (control group) or fibrin glue +PRP (PRP group). Western blot assay was used to detect neurotrophic factors secreted by PRP. Nerve regeneration was assessed by motor function, electrophysiology, and histology studies.
RESULTS
High levels of neurotrophin-3, angiopoietin-1, glial cell line derived neurotrophic factors, nerve growth factors and brain derived neurotrophic factors were demonstrated in PRP. Motor function recovery, compound motor action potentials, and axon count showed significant improvement in guinea pig treated with PRP.
CONCLUSION
There was an improved functional outcome with the use of PRP in comparison with control. The increased nerve regeneration found in this study may be due to the neurotrophic factors secreted by PRP.
MeSH Terms
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Action Potentials
Angiopoietin-1
Animals
Axons
Axotomy
Blood Platelets
Blotting, Western
Brain-Derived Neurotrophic Factor
Electrophysiology
Facial Nerve
Fibrin Tissue Adhesive
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factors
Guinea Pigs
Nerve Growth Factor
Nerve Growth Factors
Nerve Regeneration
Platelet-Rich Plasma
Recovery of Function
Regeneration
Angiopoietin-1
Brain-Derived Neurotrophic Factor
Fibrin Tissue Adhesive
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factors
Nerve Growth Factor
Nerve Growth Factors
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