Korean J Hematol.  2006 Sep;41(3):167-171. 10.5045/kjh.2006.41.3.167.

Diagnostic Usefulness of Antibody Identification Test Including Enzyme Method

• Lee DD
• Oh SH
• Shin HJ
• Chung JS
• Cho GJ
• Park TS
• Lee EY
• Son HC
• Kim HH

• KMID: 2252297
• DOI: http://doi.org/10.5045/kjh.2006.41.3.167

Abstract

BACKGROUND: Many medical institutions in Korea have recently been performing an antibody screening test as one of the essential elements of a pre-transfusion test. In this study we will determine the advantage and clinical significance of adding an enzyme method to the antiglobulin method while conducting the antibody identification test.
METHODS
We performed antibody identification tests between December 2002 and December 2005, for a total of 37 months at Pusan National University Hospital. In this study we have analyzed 550 cases that were conducted by both the antiglobulin method and the enzyme method at the same time.
RESULTS
A total of 111 of the results were cases of detection using the adding an enzyme method. Among these results, Rh antibodies that included the anti-E had the highest number of results 77 (69.4%), 28 antibody (25.2%), 2 anti-P1 (1.8%) and one anti-Jkb (0.9%).
CONCLUSION
Using the enzyme method in the antibody identification test proved to us that there were more clinically significant warm antibodies than cold antibodies. In order to have a more secured transfusion, it is required to identify a clinically significant antibody using the additional enzyme method during the antibody identification test.

Keyword

Antibody identification test; Antiglobulin method; Enzyme method

MeSH Terms

Antibodies
Busan
Korea
Mass Screening
Antibodies

Reference

1). Brecher ME. Technical manual. 15th ed.Bethesda, Maryland: American Association of Blood Banks;2005. p. 271–360.

2). Lee WH., Kim SY., Kim HO. The incidence of unexpected antibodies in transfusion candidates. Korean J Blood Transfusion. 2000. 11:99–103.

3). Cho YK., Kim DS., Lee HS., Choi SI. The frequency and distribution of unexpected antibodies in surgical patients at Chonbuk National University Hospital. Korean J Lab Med. 2004. 24:67–71.

4). Oh GY., Huh JW., Lee MA., Chung WS. Usefulness of column agglutination test for irregular antibody screening and identification. Korean J Clin Pathol. 2000. 20:98–102.

5). Jung TK., Lee NY., Bae HG., Kwon EH., Park SH., Suh JS. Unexpected antibody positivity with the Use of the LISS/Coombs gel test. Korean J Clin Pathol. 2001. 21:422–5.

6). Han KS., Han BY. Antibody Screening Test, low-ionic-strength salt solution, Unexpected Antibodies. Korean J Clin Pathol. 1994. 14:212–6.

7). Park TS., Kim KU., Kim HH, et al. Acute hemolytic transfusion reactions due to multiple alloantibodies including anti-E, anti-c and anti-Jk^b. J Korean Med Sci. 2003. 18:894–6.

8). Kim HH., Park TS., Oh SH., Lee EY., Son HC. Naturally-occurring anti-Mi^a in a 16-year-old Korean man: a case study and a review of the literature. Korean J Lab Med. 2004. 24:146–8.

9). Park TS., Hwang SY., Kim HH, et al. Transfusion therapy in a patient with hemolytic transfusion reaction due to anti-Jk^a. Korean J Blood Transfusion. 2003. 14:60–4.

10). Park TS., Oh SH., Choi JC, et al. The clinical significance of antibody screening test including di^a panel cell in Asian-mongoloid populations. J Korean Med Sci. 2003. 18:669–72.

11). Park TS., Oh SH., Choi JC, et al. A case of anti-Xg^a in a young man without previous transfusion. Korean J Lab Med. 2002. 22:437–40.

12). Sung NH., Jeon TY., Lee EY., Chung JS., Cho GJ., Kim HH. Prevalence and specificity of unexpected antibody using antibody screening test including Di^a and Mi^a cells. Korean J Lab Med. 2005. 25:340–6.

13). Shin JW., Jeong SH., Nahm CH., Kim HO., Kwon OH. The direct antiglobulin test and antibody screening test based on the antiglobulin gel technique. Korean J Clin Pathol. 1996. 16:411–8.

14). Han KS., Park MH., Kim SI. Transfusion medicine. 2nd ed.Seoul: Korea Medical Publishing Co;1999. p. 83–252. 364-6.

15). Issitt PD., Combs MR., Bredehoeft SJ, et al. Lack of clinical significance of “enzyme-only” red cell alloantibodies. Transfusion. 1993. 33:284–93.

16). Reisner R., Butler G., Bundy K., Moore SB. Comparison of the polyethylene glycol antiglobulin test and the use of enzymes in antibody detection and identification. Transfusion. 1996. 36:487–9.

17). Van Dijk BA. Lack of clinical significance of “enzyme-only” red cell alloantibodies. Transfusion. 1993. 33:960.

18). Pereira A., Mazzara R. Enzyme techniques in pre-transfusion testing. Transfusion. 1993. 33:884.

19). Abou-Jabal A., Shubeilat T., Hajjiri F. Evaluation of 2-column agglutination versus conventional tube technique for antibody screening. East Mediterr Health J. 2003. 9:407–12.

20). Walewska I., Kusnierz-Alejska G., Michalewska B. Value of the LEN (LISS-enzyme) technic in detection of alloantibodies to erythrocytes. Acta Haematol Pol. 1989. 20:214–22.

21). Hundric-Haspl Z., Jurakovic-Loncar N., Grgicevic D. Evaluation of the enzyme test for the detection of clinically significant red blood cell antibodies during pregnancy. Acta Med Croatica. 1999. 53:125–8.

22). Beadling WV., Cooling L. Immunohematology. Henry JB, editor. Clinical diagnosis and manangement by laboratory methods. 20th ed.Philadelphia: WB Saunders;2001. p. 661–717.

23). Klein HG., Anstee DJ. Mollison's blood transfusion in clinical medicine. 11th ed.Malden, Massachusetts: Blackwell Science;2005. p. 299–351.

24). Daniels G. Human blood groups. 2nd ed.Oxford: Blackwell Science;2002. p. 59–386.

25). Kwon SW., Kim DW., Han KS, et al. Annual report on external quality assessment in blood bank tests in Korea (2005). J Lab Med Quality Assurance. 2006. 28(supple 1):S111–117.