J Korean Ophthalmol Soc.
2005 Feb;46(2):360-365.
Effect of Cryotherapy on Focal Adhesion Kinase in Retinal Pigment Epithelial Cells
- Affiliations
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- 1Seoul Eye Clinic, Seoul, Korea.
- 2Department of Ophthalmology, Seoul Municipial Boramae Hospital, Seoul, Korea.
- 3Department of Ophthalmology, Seoul National University, College of Medicine, Seoul Artificial Eye Center, Seoul National University Hospital, Clinical Research Institute, Seoul, Korea. chungh@snu.ac.kr
Abstract
- PURPOSE
In the pathogenesis of proliferative vitreoretinopathy (PVR), retinal pigment epithelial (RPE) cells do not undergo apoptosis after separation from the basement membrane. In order to investigate the mechanism by which RPE cells resist apoptosis in PVR, the expression of focal adhesion kinase after cryotherapy was studied. METHODS: After lensectomy and vitrectomy was performed on rabbit eyes, a retinal tear was created. Cryotherapy was applied to the tear site one week after the tear creation. Rabbits were sacrificed at 1, 4 and 7 days after the tear formation and 30 minutes, 1, 4, 7, 9, 21 days after cryotherapy. Eyes were stained with anti-focal adhesion kinase (FAK) antibody. RESULTS: Expression of FAK increased after the tear formation and remained increased until 21days after cryotherapy. CONCLUSIONS: These data show that creation of a retinal tear and cryotherapy increases FAK expression and may contribute to resistance to apoptosis.
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