J Korean Orthop Res Soc.  2008 Oct;11(2):84-91.

Gene Expression Modulation in Rat Skeletal Muscle by Denervation-induced Atrophy

• Wang KB
• Ahn JM
• Kim HT
• Kim CM
• Suh JT

Abstract

PURPOSE
To understand the modulation of genes by atrophy, differential expression of genes in normal and denervated skeletal muscle was investigated by DNA chip technology.
MATERIALS AND METHODS
Sciatic nerve and femoral nerve were resected in right leg of rat to make the muscle atrophy model. Muscle tissues from the gastrocnemius of normal and denervated legs were homogenized and RNA were extracted. Dyes were labelled during reverse transcription and hybridization was done into the DNA chip which is consisted of about 5,000 probes.
RESULTS
By statistical analysis, 39 genes were selected as differentially expressed genes by atrophy. 15 known genes up-regulated by atrophy were genes related to immune response, extracellular matrix, andsignal transduction in plasma membrane. 7 known genes down-regulated by atrophy were genes related to cell growth and proliferation, intracellular signal transduction, and energy metabolism. Some unknown gene functions were analysed by bioinformatics analysis and they were highly homologous genes with McKusick-Kaufman syndrome protein, ADP-ribosylation factor-like 4, and component X of pyruvate dehydrogenase complex.
CONCLUSION
These results suggest that reduction of energy metabolism, activation of cholesterol exclusion, and changes on signal transduction pathway are involved in the process of atrophy by denervation in skeletal muscle of rat.

Keyword

Nerve injury; Skeletal muscle atrophy; Expressed genes

MeSH Terms

Abnormalities, Multiple
Animals
Atrophy
Cell Membrane
Chimera
Cholesterol
Coloring Agents
Computational Biology
Denervation
Energy Metabolism
Extracellular Matrix
Femoral Nerve
Gene Expression
Heart Defects, Congenital
Hydrocolpos
Leg
Muscle, Skeletal
Muscles
Muscular Atrophy
Oligonucleotide Array Sequence Analysis
Oxidoreductases
Polydactyly
Pyruvic Acid
Rats
Reverse Transcription
RNA
Sciatic Nerve
Signal Transduction
Uterine Diseases
Abnormalities, Multiple
Cholesterol
Coloring Agents
Heart Defects, Congenital
Hydrocolpos
Oxidoreductases
Polydactyly
Pyruvic Acid
RNA
Uterine Diseases