The change of bone formation according to magnetic intensity of magnet placed into titanium implant specimens

Hwang, Yun Tae; Lee, Sung Bok; Choi, Dae Gyun; Choi, Boo Byung

Abstract

PURPOSE
The purposes of this investigation were to discover the possibility of clinical application in the areas of dental implants and bone grafts by investigating the bone formation histologically around specimen which was depending on the intensity of magnetic field of neodymium magnet inside of the specimens. MATERIAL AND METHOD: 1. Measurement of magnetic intensity - placed the magnet inside of the specimen, and measured the intensity of magnetic field around the 1st thread and 3rd thread of specimen 20 times by using a Gaussmeter(Kanetec Co., Japan). 2. Surgical Procedure - Male rabbit was anesthetised by constant amount of Ketamine (0.25ml/kg) and Rompun (0.25ml/kg). After incising the flat part of tibia, and planted the specimens of titanium implant, control group was stitched without magnet, while experimental groups were placed a magnedisc 500(Aichi Steel Co., Japan) or magnedisc 800(Aichi Steel Co., Japan) into it, fixed by pattern resin and stitched. 3. Management after the surgery. - In order to prevent it from the infection of bacteria and for antiinflammation, Gentamycin and Ketopro were injected during 1 week from operation day, and dressed with potadine. 4. Preparation of histomorphometric analysis - At 2, 4 and 8 weeks after the surgery, the animals were sacrificed by excessed Ketamine, and then, specimens were obtained including the operated part and some parts of tibia, and fixed it to 10% of PBS buffer solution. After embedding specimens in Technovit 1200 and B.P solution, made a H-E stain. Samples width was 75micrometer. In histological findings through the optical microscope and using Kappa image base program(Olympus Co. Japan), the bone contact ratio and bone area ratio of each parts of specimens were measured and analyzed. 5. Statistical analysis Statistical analysis was accomplished with Mann Whitney U-test. RESULTS AND CONCLUSION: 1. In histomorphometricl findings, increased new bone formation was shown in both control and experimental groups through the experiment performed for 2, 4, and 8 weeks. After 4 weeks, more osteoblasts and osteoclasts with significant bone remodeling were shown in experimental groups. 2. In histomorphometric analysis, the bone contact ratios were 38.5% for experimental group 1, 29.5% for experimental group 2 and 11.9% for control group. Experimental groups were higher than control group(p<0.05)(Fig. 6, Table IV). The bone area ratios were 60.9% for experimental group 2, 46.4% for experimental group 1 and 36.0 % for control group. There was no significantly ststistical difference between experimental groups and control group(p<0.05)(Fig. 8, Table VII) 3. In comparision of the bone contact ratios at each measurement sites according to magnetic ntensity, experimental group 2(5.6mT) was higher than control group at the 1st thread (p<0.05) and experimental group 1(1.8mT) was higher than control group at the 3rd thread(p<0.05)(Fig. 7, Table V, VI). 4. In comparision of the bone area ratios at each measurement sites according to magnetic intensity, experimental group 2(5.6mT) was higher than control group and experimental group 1(4.0mT) at the 1st thread(p<0.1) and experimental group 2(4.4mT) was higher than experimental group 1(1.8mT) at the 3rd thread(p<0.1)(Fig. 9, Table IX, X). Experiment group 2 was largest, followed by experiment group1 and control group at the 3rd thread of implant. There was a significant difference at the 1st thread of control group and experiment group 2. and at 1st thread and 3rd thread of experiment group 1 and 2, and not at control group experiment group 1.(p<0.1)