Korean Circ J.  2001 Dec;31(12):1252-1260. 10.4070/kcj.2001.31.12.1252.

Effect of Preconditioning by Low-Flow Perfusion on Cardioprotection

• Kim HD

• KMID: 1938914
• DOI: http://doi.org/10.4070/kcj.2001.31.12.1252

Abstract

BACKGROUND AND OBJECTIVES
Recent reports suggest that brief periods of low-flow ischemia (lf Isc) and reperfusion (R) before prolonged Isc mimic ischemic preconditioning (IPC) in murine hearts, probably by a preservation of high energy phosphates. MATERIALS AND METHODS: In order to test this hypothesis, Langendorff-perfused isolated rabbit hearts were subjected to 45 min lf Isc (5% of baseline perfusion flow) with lf IPC or not, followed by 120 min R. lf IPC was induced by a single episode of 5 min lf Isc and 10 min R. These were compared with IPC hearts by 5 min no-flow Isc and 10 min R.
RESULTS
lf IPC as well as IPC enhanced post-ischemic functional recovery although IPC did not reduce infarct size (Isc control, 37.5+/-3.1, IPC, 16.2+/-1.5, lf Isc, 43.0+/-0.7, and lf IPC, 40.4+/-1.0% of the left ventricle). Myocardial ATP hydrolysis and lactate production during the preischemic, ischemic, and reperfusion periods were not differ between the experimental groups.
CONCLUSION
These results suggest that a brief period of lf Isc could not precondition the rabbit heart and the energy metabolism hypothesis may not be a universal mechanism for the cardioprotective effect of IPC.

Keyword

Ischemic preconditioning; Myocardial infarction; Phosphates; Lactic acid

MeSH Terms

Adenosine Triphosphate
Energy Metabolism
Heart
Hydrolysis
Ischemia
Ischemic Preconditioning
Lactic Acid
Myocardial Infarction
Perfusion*
Phosphates
Reperfusion
Adenosine Triphosphate
Lactic Acid
Phosphates