J Periodontal Implant Sci.  2010 Dec;40(6):265-270. 10.5051/jpis.2010.40.6.265.

Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells

• Kim HS
• Kim KH
• Kim SH
• Kim YS
• Koo KT
• Kim TI
• Seol YJ
• Ku Y
• Rhyu IC
• Chung CP
• Lee YM

• KMID: 1783570
• DOI: http://doi.org/10.5051/jpis.2010.40.6.265

Abstract

PURPOSE
The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro.
METHODS
Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method.
RESULTS
Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups.
CONCLUSIONS
Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.

Keyword

Allogenic; Immunomodulation; Periodontal ligaments; Stem cell; Xenogenic

MeSH Terms

Apoptosis
Bone Marrow
Cell Culture Techniques
Cell Division
Diminazene
Fluoresceins
Humans
Immunomodulation
Ligaments
Periodontal Ligament
Stem Cells
Succinimides
Trypan Blue
Diminazene
Fluoresceins
Succinimides
Trypan Blue

Figure

• 

  Figure 1 Inhibition of the proliferation of allogenic and xenogenic peripheral blood mononuclear cells (PBMCs) when co-cultured with periodontal ligament stem cells (PDLSCs) and bone marrow stem cells (BMSCs), as evaluated in MTS assays. (A) Allogenic mixed cell cultures (canine stem cells+allogenic canine PBMCs). (B) Xenogenic mixed cell cultures (canine stem cells+xenogenic human PBMCs). cPBMCs: canine PBMCs, hPBMCs: human PBMCs, cPDLSCs: canine PDLSCs, cBMSCs: canine BMSCs. *Significantly different from negative control (P<0.05).

• 

  Figure 2 The cell division analysis of allogenic and xenogenic peripheral blood mononuclear cells (PBMCs) when co-cultured with periodontal ligament stem cells and bone marrow stem cells, evaluated using CFSE assays. (A) Allogenic mixed cell cultures (canine stem cells+allogenic canine PBMCs). (B) Xenogenic mixed cell cultures (canine stem cells+xenogenic human PBMCs). cPBMC: canine PBMC, hPBMC: human PBMC, cPDLSC: canine periodontal ligament stem cell, cBMSC: canine bone marrow stem cell.

• 

  Figure 3 Trypan blue uptake methods for the assessment of apoptosis of peripheral blood mononuclear cells (PBMCs). (A) Allogenic mixed cell cultures (canine stem cells+allogenic canine PBMCs). (B) Xenogenic mixed cell cultures (canine stem cells+xenogenic human PBMCs). In all groups, no significant differences in the percentages of trypan blue positive PBMCs were found. cPBMC: canine PBMC, hPBMC: human PBMC, cPDLSC: canine periodontal ligament stem cell, cBMSC: canine bone marrow stem cell.

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