Korean J Lab Med.
2003 Oct;23(5):357-362.
Evaluation of Sensitivity and Specificity of MAGE A1-6 RT-nested PCR as a Cancer Detection Method
- Affiliations
-
- 1Department of Laboratory Medicine, School of Medicine, Catholic University of Daegu, Korea. chjeon@cu.ac.kr
- 2Department of Internal Medicine, School of Medicine, Catholic University of Daegu, Daegu, Korea.
- 3Department of Laboratory Medicine, Korean Cancer Center, Seoul, Korea.
- 4Department of Otolaryngology, School of Medicine, Kosin University, Busan, Korea.
- 5Department of Internal Medicine, School of Medicine, Kosin University, Busan, Korea.
- 6Department of Pathology, School of Medicine, Kosin University, Busan, Korea.
- 7Department of Thoracic Surgery, School of Medicine, Keimyung University, Daegu, Korea.
- 8Department of Immunology, School of Medicine, Keimyung University, Daegu, Korea.
Abstract
- BACKGROUND
The aim of this study is to evaluate the sensitivity and specificity of MAGE (Melanoma Antigen Gene) A1-6 RT-nested PCR as a detection method for cancer cells. METHODS: From February 2001 and December 2002, various samples [cancer tissue, sputum, induced sputum, broncho-alveolar lavage (BAL), blood, urine and cervical brush] were obtained from 355 cancer patients. Moreover, 316 samples, including cancer adjacent normal tissue, blood, urine and sputum were collected for the negative controls. MAGE A1-6 RT-nested PCR was performed and the results were evaluated with clinical diagnosis. The sensitivity of the MAGE assay was compared with that of the telomerase assay and cytologic examination using 26 sputa of lung cancer patients. RESULTS: The average sensitivity of the MAGE assay in cancer tissues was 79% (85% in squamous carcinoma and 70% in adenocarcimona). The sensitivity in body fluids was 57% (9-27% in blood, 59% in sputum, 68% in urine, more than 70% in induced sputum and BAL, and 87% in pleural fluid and cervical brush). In the negative controls, the positive rates were less than 8.9% in normal tissues and 2% in blood, sputum and urine. The average sensitivity of the MAGE RT-PCR, telomerase assay and cytologic examination was 61.5%, 26.9% and 15.4%, respectively. CONCLUSIONS: The MAGE A1-6 RT-PCR showed excellent sensitivity and specificity. Therefore, it could be effectively utilized as a cancer detection method in the clinical laboratory.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid
- MAGE Gene Expression in Bronchial Washing Fluid in Suspected Parenchymal Lung Cancer
- The Clinical Implication of MAGE Gene Detection in Bronchial Washing Fluid in Routine Practice
- Detection of Rare Cancer Cells in the Blood by RNA Extraction of Filtered Mononuclear Cells and Reverse Transcription-PCR
- The expression of melanoma antigen encoding gene in lung cancer
