Korean J Parasitol.  2014 Feb;52(1):41-46. 10.3347/kjp.2014.52.1.41.

Diagnostic Efficacy of a Recombinant Cysteine Protease of Spirometra erinacei Larvae for Serodiagnosis of Sparganosis

Affiliations
  • 1Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center, Seoul 110-799, Korea. mhchoi@snu.ac.kr

Abstract

The mature domain of a cysteine protease of Spirometra erinacei plerocercoid larva (i.e., sparganum) was expressed in Escherichia coli, and its value as an antigen for the serodiagnosis of sparganosis was investigated. The recombinant protein (rSepCp-1) has the molecular weight of 23.4 kDa, and strongly reacted with the sparganum positive human or mice sera but not with negative sera by immunoblotting. ELISA with rSepCp-1 protein or sparganum crude antigen (SeC) was evaluated for the serodiagnosis of sparganosis using patient's sera. The sensitivity and specificity of ELISA using rSepCp-1 protein were 95.0% (19/20) and 99.1% (111/112), respectively. In contrast, the sensitivity and specificity of ELISA with SeC were 100% (20/20) and 96.4% (108/112), respectively. Moreover, in experimentally infected mice, the sensitivity and specificity of both ELISA assays were 100% for the detection of anti-sparganum IgG. It is suggested that the rSepCp-1 protein-based ELISA could provide a highly sensitive and specific assay for the diagnosis of sparganosis.

Keyword

Spirometra erinacei; sparganum; cysteine protease; ELISA; serodiagnosis

MeSH Terms

Animals
Antigens, Helminth/chemistry/*diagnostic use/genetics
Cloning, Molecular
Cysteine Proteases/chemistry/*diagnostic use/genetics
Enzyme-Linked Immunosorbent Assay
Escherichia coli/genetics
Gene Expression
Humans
Mice
Molecular Weight
Parasitology/*methods
Recombinant Proteins/chemistry/diagnostic use/genetics
Sensitivity and Specificity
Serologic Tests/methods
Sparganosis/*diagnosis
Spirometra/*enzymology/genetics
Antigens, Helminth
Cysteine Proteases
Recombinant Proteins
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