The effect of implant surface treated by anodizing on proliferation of the rat osteoblast

Hur, Yin Shik; Park, Joon Bong; Kwon, Young Hyuk; Herr, Yeek; Kim, Hyung Sun; Cho, Byung Won; Cho, Won Il

Abstract

The surface characteristics of titanium have been shown to have an important role in contact ossseointegration around the implant. Anodizing at high voltage produces microporous structure and increases thickness of surface titanium dioxide layer. The aim of present study was to analyse the response of rat calvarial osteoblast cell to commercially pure titanium and Ti-6Al-4V anodized in 0.06 mol/l beta-glycerophosphate and 0.03 mol/l sodium acetate. In this study, rat calvarial osteoblasts were used to assay for cell viability and cell proliferation on the implant surface at 1, 2, 4, 7 days. 1. Surface roughness was 1.256micrometer at 200V, and 1.745micrometer at 300V. 2. The thickness of titanium oxide layer was increased 1micrometer with the increase of 50V. 3. The proliferation rate of osteoblastic cells was increased with the increase of the surface roughness and the thickness of titanium oxide layer. 4. There was no difference in cell viability and cell proliferation between commercially pure titanium and Ti- 6Al-4V anodized at the same condition. In conclusion, the titanium surface modified by anodizing was biocompatible, produced enhanced osteoblastic response. The reasons of enhanced osteoblast response might be due to reduced metal ion release by thickened and stabilized titanium dioxide layer and microporous rough structures.