Korean J Clin Microbiol.  2012 Sep;15(3):114-116. 10.5145/KJCM.2012.15.3.114.

Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea

• Park KS
• Ki CS
• Lee NY

• KMID: 1434170
• DOI: http://doi.org/10.5145/KJCM.2012.15.3.114

Abstract

Blastomycosis, endemic in North America, has been hardly reported in Korea. We describe laboratory experience in phenotypic and molecular identification of Blastomyces dermatitidis first isolated in Korea. The patient was a 45-year-old male with pulmonary blastomycosis mimicking pulmonary tuberculosis. Diagnosis was based on culture and dimorphism combined with DNA target sequencing of internal transcribed spacers (ITS) and D1/D2 regions.

Keyword

Blastomyces dermatitidis; Blastomycosis; D1/D2; Internal transcribed spacers (ITS); Molecular identification

MeSH Terms

Blastomyces
Blastomycosis
DNA
Humans
Korea
Male
Middle Aged
North America
Tuberculosis, Pulmonary
DNA

Figure

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  Fig. 1 (A) White, cottony colonies on the front side of Sabouraud's dextrose agar (incubated at 30℃ for 10 days). (B) Tan, wrinkled colonies on the reverse side of Sabouraud's dextrose agar (incubated at 30℃ for 10 days). (C) Septate hyphae with round, lollipop-like conidia which are developing at the apex of the conidiophores (incubated at 30℃ for 10 days, lactophenol cotton blue stain, ×400).

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  Fig. 2 (A) Cream-colored colonies on brain heart infusion (BHI) agar (incubated at 35℃ for 12 days). (B) Yeastlike cells with characteristic thick, double contoured walls and broad-based buds (incubated at 35℃ for 12 days, lactophenol cotton blue stain, ×400).

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