Int J Oral Biol.  2013 Jun;38(2):81-85.

Antibacterial Activity of Hydrogen-rich Water Against Oral Bacteria

Affiliations
  • 1Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan, Korea. micro94@gmail.com

Abstract

There are estimated to be about 700 species of bacteria in the oral cavity. Based on epidemiological investigations, some of these strains have been proposed as the pathogens responsible for oral diseases such as dental caries, gingivitis and periodontitis. Since electrolyzed hydrogen-rich water has been shown to have beneficial effects on human immunity, its use has increased. In our study, the antibacterial activity of hydrogen-rich water for oralagainst bacteria associated with oral disease was evaluated. The bacterial strains Streptococcus mutans, Fusobacterium nucleatum, Porphyromonas gingivalis and Tannerella forsythia were cultured in specific growth medium. S. mutans, F. nucleatum and P. gingivalis were soaked to thein both hydrogen water and tap water for 30 sec and then inoculated onto mitis-salivarius agar and brain heart infusion agar including supplemented withvitamin K and hemin, respectively. The numbers of bacterial colonies were then measured after cultivation for 48 hours. In the case of T. forsythia, which does not grow well on agar plates, inoculations into modified new oral spirochete (NOS) broth were performed and growth curve analysis was undertaken every day with a spectrophotometer. Hydrogen water showed antibacterial activity against all four bacterial strains in comparison with tap-water. We conclude from this that hydrogen water may have a positive impact on oral hygiene by helping to remove cariogenic bacteria and periodontopathogens.

Keyword

Hydrogen-rich water; Antibacterial activity; Oral bacteria

MeSH Terms

Agar
Bacteria
Brain
Dental Caries
Forsythia
Fusobacterium nucleatum
Gingivitis
Heart
Hemin
Humans
Hydrogen
Mouth
Oral Hygiene
Periodontitis
Porphyromonas gingivalis
Spirochaetales
Streptococcus mutans
Water
Agar
Hemin
Hydrogen
Water
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