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Purification and cloning of glyoxalase II from rat liver

Cho MY, Bae CD, Park JB, Lee TH

  • KMID: 754988
  • Exp Mol Med.
  • 1998 Mar;30(1):53-57.
Glyoxalase (GLO) II, which is a component of GLO system and catalyze the conversion of S-lactoyl-glutathione to D-lactate, was purified 1488 fold from rat liver by two steps of Affigel blue and...
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Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti

Kang SW, Choi EJ, Kweon CH

The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned...
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TRAPR: R Package for Statistical Analysis and Visualization of RNA-Seq Data

Lim JH, Lee SY, Kim JH

High-throughput transcriptome sequencing, also known as RNA sequencing (RNA-Seq), is a standard technology for measuring gene expression with unprecedented accuracy. Numerous bioconductor packages have been developed for the statistical analysis...
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Molecular cloning of a rhoptry protein (ROP6) secreted from Toxoplasma gondii

Ahn HJ, Kim S, Nam HW

Monoclonal antibody (mAb) Tg786 against Toxoplasma gondii has been found to detect a 42-kDa rhoptry protein (ROP6) which showed protease activity and host cell binding characteristics after secretion. Using the...
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Cloning a new allele form of bovine TNF-alpha

Ahn J

  • KMID: 1103522
  • J Vet Sci.
  • 2001 Aug;2(2):139-141.
Although little is known on the function of gammadelta T lymphocytes, there is increasing evidence that gammadelta T lymphocytes are early responders and modulators of immune responses against pathogens and...
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Molecular cloning and sequencing of rat Cdc42 GTPase cDNA

Han JS, Kim JH, Kim JG, Park JB, Noh DY, Lee KH

  • KMID: 755074
  • Exp Mol Med.
  • 2000 Sep;32(3):115-119.
Cdc42 is a member of the Rho family of small GTP-ase and plays an important role in intracellular signaling pathways regulating cell morphology, motility and stimulation of DNA...
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Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase 2

Lee SY, Chung JY, Shin IS, Choi EW, Hwang CY, Youn HY, Han HR

  • KMID: 1114855
  • J Vet Sci.
  • 2005 Jun;6(2):141-145.
The research of p53 is being conducted to find the mechanisms of tumorigenesis and to treat various cancers. Homeodomain-interacting protein kinase2 (HIPK2) is an important factor to regulate p53 and...
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Cloning of BNIP3h, a member of proapoptotic BNIP3 family genes

Farooq M, Kim YH, Im SU, Chung EJ, Hwang SY, Sohn MY, Kim MK, Kim JC

  • KMID: 938882
  • Exp Mol Med.
  • 2001 Sep;33(3):169-173.
Apoptosis is regulated by interaction of antiapoptotic Bcl-2 family proteins with various proapoptotic proteins, several of which are also members of the Bcl-2 family. BNIP3 (formerly NIP3) is a proapoptotic...
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Whole Mitochondrial Genome Sequence of an Indian Plasmodium falciparum Field Isolate

Tyagi S, Pande V, Das A

Mitochondrial genome sequence of malaria parasites has served as a potential marker for inferring evolutionary history of the Plasmodium genus. In Plasmodium falciparum, the mitochondrial genome sequences from around the...
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A variant of ornithine aminotransferase from mouse small intestine

Lim SN, Rho HW, Park JW, Jhee EC, Kim JS, Kim HR

  • KMID: 1109295
  • Exp Mol Med.
  • 1998 Sep;30(3):131-135.
The ornithine aminotransferase (OAT) activity of mouse was found to be highest in the small intestine. The mitochondrial OAT from mouse small intestine was purified to homogeneity by the procedures including heart...
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Molecular Variation and Distribution of Anopheles fluviatilis (Diptera: Culicidae) Complex in Iran

Naddaf SR, Razavi MR, Bahramali

Anopheles fluviatilis James (Diptera: Culicidae) is one of the known malaria vectors in south and southeastern Iran. Earlier ITS2 sequences analysis of specimens from Iran demonstrated only a single genotype...
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Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant

Kim JR, Kim SY, Kim JH

  • KMID: 1109297
  • Exp Mol Med.
  • 1998 Sep;30(3):145-149.
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR)...
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Divergent long-terminal-repeat retrotransposon families in the genome of Paragonimus westermani

Bae YA, Kong Y

To gain information on retrotransposons in the genome of Paragonimus westermani, PCR was carried out with degenerate primers, specific to protease and reverse transcriptase (rt) genes of long-terminal-repeat (LTR) retrotransposons....
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Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase

Sun H, Wolfe JH

  • KMID: 755097
  • Exp Mol Med.
  • 2000 Dec;32(4):187-192.
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species...
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Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains

Hartati S, Kusumawati A, Wuryastuti H, Widada JS

Toxoplasma gondii is a persistent protozoan parasite capable of infecting almost any warm-blooded vertebrates. SAG1 (p30) is the prototypic member of a superfamily of surface antigens called SRS (SAG1-related sequence)....
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Aspartic proteases of Plasmodium vivax are highly conserved in wild isolates

Na BK, Lee EG, Lee HW, Cho SH, Bae YA, Kong Y, Lee JK, Kim TS

The plasmepsins are the aspartic proteases of malaria parasites. Treatment of aspartic protease inhibitor inhibits hemoglobin hydrolysis and blocks the parasite development in vitro suggesting that these proteases might be...
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Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidae)

Lee SU, Huh S

We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening...
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Molecular cloning of ribosomal P protein in Toxoplasma gondii and the availability to detect antibody against recombinant protein in toxoplasmosis patients

Ahn HJ, Kim S, Nam HW

Among the panel of monoclonal antibodies (mAb) against Toxoplasma gondii, mAb of Tg621 (Tg621) clone blotted 38 kDa protein which localized in the cytoplasm of tachyzoites by immunofluorescence microscopy. The...
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Cloning and characterization of 5'-upstream region of human phospholipase C-beta2 gene

Yun ES, Lee SJ, Kim MJ, Ryu SH, Suh PG

  • KMID: 1085743
  • Exp Mol Med.
  • 2001 Jun;33(2):76-82.
5'-upstream region of the phospholipase C-beta2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284...
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Genomic structure of the regulatory region of the voltage-gated calcium channel alpha 1D

Kim HL, Chang YJ, Lee SM, Hong YS

  • KMID: 755016
  • Exp Mol Med.
  • 1998 Dec;30(4):246-251.
In excitable and endocrine organs, calcium influxes through the L-type voltage-gated calcium channel (VGCC) which is composed of four (alpha 1, alpha 2, beta, and gamma) subunits. Temporal and spatial...
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