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Epidemiological Analysis of Shiga Toxin-producing E. coli Isolated in Gwangju, Korea, by Pulse-field Gel Electrophoresis

Lee JC, Kim MJ

In Gwangju, Korea, over the last 4 years, human gastrointestinal infection caused by shiga toxin-producing E. coli (STEC) increased. The aim of this study was to ascertain the genetic relatedness...
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Molecular Epidemiology of Legionella pneumophila Isolated from Bath Facilities of Public Establishments in Seoul

Jeon SJ, Jung JH, Jin YH, Lee JK, Oh YH, Choi SM

The genus Legionella is common in aquatic environments. Some species of Legionella are recognized as potential opportunistic pathogens for human, notably Legionella pneumophila that causes Legionellosis. During the summer season...
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Genomic analysis of Mycobacterium foruitum by pulsed-filed gel electrophoresis

Lee TY, Do IA, Kim SK

Epidemiological studies are important in both the prevention and treatment of mycobacterial infections. This study was initiated to establish the pulsed-field gel electrophoresis (PFGE) method, which are not yet extensively...
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Molecular Strain Typing of Legionella Isolates from Water in Cooling Towers of Big Buildings in Busan, Korea

Kim JM, Jeong SH, Seo D, Park EH, Song EJ, Choi JC, Lee EY, Chang CL

  • KMID: 2089697
  • Korean J Clin Microbiol.
  • 2004 Mar;7(1):38-42.
BACKGROUND: It is important to define a source of infection when outbreak of Legionella infections has occurred. The performance of a molecular strain typing method was evaluated for environmental and...
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Emerging antimicrobial resistance, plasmid profile and pulsed-field gel electrophoresis pattern of the endonuclease-digested genomic DNA of Neisseria gonorrhoeae

Lee K, Shin JW, Lim JB, Kim YA, Yong D, Oh HB, Chong Y

Resistant gonococci are very prevalent in many countries, particularly in Asia. This study was conducted to determine the trend of resistance, the effect of decreasing the ciprofloxacin susceptibilities of gonococci...
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Multilocus Sequence Typing for Candida albicans Isolates from Candidemic Patients: Comparison with Southern Blot Hybridization and Pulsed-field Gel Electrophoresis Analysis

Myoung Y, Shin JH, Lee JS, Kim SH, Shin MG, Suh SP, Ryang DW

BACKGROUND: We evaluated the efficacy of multilocus sequence typing (MLST) for assessing the genetic relationship among Candida albicans isolates from patients with candidemia in a hospital setting. METHODS: A total of...
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Pulsed-Field Gel Electrophoresis Analysis of a Salmonella london Outbreak in Neontes

Kim KY, Lee HK, Cho SR

  • KMID: 2144574
  • J Korean Soc Neonatol.
  • 2001 Nov;8(2):222-228.
PURPOSE: Salmonella species is one of the most common causative organisms of acute gastroenteritis in neonates. There have been some reports of outbreaks of Salmonella species in neonates, but none...
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Pseudo-outbreak of Brevundimonas diminuta

Kim YH, Koh EM, Lee YW, Lee YK, Shin HB, Lee K

Brevundimonas diminuta is a lactose non-fermenting Gram-negative rod associated with infection in immunocompromised patients. In three patients from two general wards, B. diminuta was isolated in blood culture sample. The...
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Molecular Typing in Public Health Laboratories: From an Academic Indulgence to an Infection Control Imperative

Allerberger F

Using three Austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction...
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Pulsed-Field Gel Electrophoresis (PFGE) Patterns and Antibiotypes for Sequential Isolates of Staphylococcus epidermidis from Blood Cultures

Choi IO, Jung SI, Shin DH, Park YK, Shin JH

  • KMID: 2285108
  • Infect Chemother.
  • 2004 Oct;36(5):279-285.
BACKGROUND: The significance of Staphylococcus epidermidis positive blood cultures is difficult to determine, but repeated isolation of the same organism with the same genotype is suggestive of true bacteremia. MATERIALS...
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Emergence of optrA-Mediated Linezolid-Nonsusceptible Enterococcus faecalis in a Tertiary Care Hospital

Park K, Jeong YS, Chang J, Sung H, Kim MN

This study investigated resistance mechanisms and epidemiology of emerging linezolid-nonsusceptible Enterococcus faecalis (LNSEF) in a tertiary care hospital. LNSEF isolated from clinical samples were collected from November 2017 to June...
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Epidemiologic Investigation of an Outbreak of Serratia marcescens Urinary Tract Infection in an Intensive Care Unit Using Pulsed-Field Gel Electrophoresis

Koh EH, Kim S, Bae IG

  • KMID: 2224690
  • Korean J Clin Microbiol.
  • 2005 Apr;8(1):34-40.
BACKGROUND: Serratia marcescens is a well-known cause of nosocomial infections. We investigated an outbreak of S.marcescens infections in a surgical intensive care unit (SICU) and identified the source of the...
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Recurrent Pseudomonas aeruginosa Infection in Chronic Lung Diseases: Relapse or Reinfection?

Yum HK, Park IN, Shin BM, Choi SJ

BACKGROUND: Pseudomonas aeruginosa infection is particularly associated with progressive and ultimately chronic recurrent respiratory infections in chronic obstructive pulmonary disease, bronchiectasis, chronic destroyed lung disease, and cystic fibrosis. Its treatment...
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Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE)

Byun JH, Yoo JH, Park C, Lee DG, Park SH, Choi SM, Choi JH, Kim SH, Kwon JC

BACKGROUND: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli, and also assessed the...
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Comparison of Pulsed-field Gel Electrophoresis, Amplified Fragment Length Polymorphism, and Infrequent Restriction Site-Polymerase Chain Reaction for Molecular Typing of Escherichia coli and Staphylococcus aureus Strains

Kim SI, Yoo JH, Cho YK, Lee DG, Wie SH, Choi JH, Kim YR, Shin WS, Kang MW

  • KMID: 2147930
  • Korean J Infect Dis.
  • 1999 Dec;31(6):474-480.
BACKGROUND: Pulsed-field gel electrophoresis (PFGE) has been regarded a standard method for genotyping in epidemiologic studies. However, it is tedious and time-consuming to perform. Two alternative genotyping methods have recently...
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Comparison of the Usefulness of Pulsed-Field Gel Electrophoresis and Ribotyping in Epidemiological Study of Methicillin-Resistant Staphylococcus aureus

Park JI, Jun JH, Kim HR, Lee JN

  • KMID: 2300535
  • Korean J Clin Pathol.
  • 1998 Dec;18(4):577-583.
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most frequent agents of hospital infections. The aim of this study was to evaluate the polymorphism of MRSA strains from our...
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Clonality Analysis Using Pulsed-Field Gel Electrophoresis of Erythromycin Resistant Group A Streptococci

Lee NY, Koh EH, Kim S

  • KMID: 2089695
  • Korean J Clin Microbiol.
  • 2004 Mar;7(1):27-30.
BACKGROUND: Antibiotic resistance of group A streptococci (GAS) is increasing nationwide. Pulsed-field gel electrophoresis (PFGE) is useful for investigating genetic relationship among outbreaks of bacterial infection. Erythromycin (EM) resistance is...
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Comparison of infrequent restriction site-polymerase chain reaction and pulsed-field gel electrophoresis for molecular typing of Staphylococcus aureus and Escherichia coli

Shin WS, Kim TG, Choi JH, Lee DG, Choi HB, Yoo JH, Kim JH, Kang JH, Min WS

  • KMID: 2146873
  • J Korean Soc Microbiol.
  • 2000 Aug;35(4):289-297.
BACKGROUND: Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are major pathogens in community and hospital. And they sometimes cause the outbreak in hospital in the immunocompromised patients. Pulsed-field gel electrophoresis (PFGE)...
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Typing and Antimicrobial Susceptibilities of Methicillin Resistant Staphylococcus aureus (MRSA) Strains Isolated in a Hospital in Korea

Lee HJ, Suh JT, Kim YS, Lenz W, Bierbaum G, Schaal KP

Methicillin-resistant Staphylococcus aureus (MRSA) strains may cause serious nosocomial infections, including pneumonia and septicemia. The rate of methicillin-resistance among S. aureus isolates in Korea is over 50%. In this study,...
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Comparison of serotypes, restriction enzyme analysis of plasmid DNA pattern and PFGE(pulsed-field gel electrophoresis) patterns of Yersinia pseudotuberculosis isolates in Korea.

Kim MA, Cho CR, Paik IK, Park JI, Lee JN

  • KMID: 2225903
  • Korean J Clin Pathol.
  • 1999 Dec;19(6):689-695.
BACKGROUND: Yersinia pseudotuberculosis, a member of genus Enterobactericeae, is a main etiologic organism of diarrhea in childhood. Because a mouse and a unchlorinated spring water are main reservoirs of Y....
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