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Study on isolation of Prevotella nigrescens 9336-specific DNA probes using random cloning method

Gang SW, Kim SH, Kim DK, Seong JH, Kim BO, Han JJ, Kook JK

The purpose of this study is to develop species-specific DNA probes and polymerase chain reaction (PCR) primers for detection and identification of Prevotella nigrescens (P. nigrescens) 9336. This study procedure...
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Detection of Human Papillomavirus DNA by In Situ Hybridization Using Biotinylated DNA Probes in Codylomata Acuminata

Kim SJ, Lee KS, Song JY, Suh SI, Suh MH

  • KMID: 1920691
  • Korean J Dermatol.
  • 1990 Dec;28(6):702-707.
Human Papillomaviruses(HPVs) are regarded as sexually transmitable and closely associated with precancerous lesions or invasive cancers of the vulva, vagina, and cer V1X. In situ hybridization, besides showing higher sensitivity,...
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Molecular Genetic Study on Human Meningiomas:Loss of Heterozygosity on Chromosome 22

Kim JH, Lee SH, Park SY, Lee JH

  • KMID: 2138513
  • J Korean Neurosurg Soc.
  • 1991 Apr;20(4):193-198.
With the methods of restriction fragment length polymorphisms(RFLPs) and Southern blot, we performed a molecular genetic study in 20 cases of intracranial meningiomas. There were four cases of invasive meningiomas,...
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Studies on the bfp Gene, Adherence to HEp-2 Cells and Serotyping of Escherichia coli Isolated from Urine

Kim YB, Kim MJ, Park GS

  • KMID: 2146940
  • J Korean Soc Microbiol.
  • 1998 Feb;33(1):77-87.
Eighty-two strains of Escherichia coli isolated from urine specimens in Pusan University Hospital, were serotyped and analyzed for plasmid DNA profiles, PFGE profiles, MRHA of human blood cells, HEp-2 cell...
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Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization

Park SN, Kook JK

  • KMID: 1431092
  • Int J Oral Biol.
  • 2013 Jun;38(2):43-49.
The objective of this study was to develop PCR primers that are specific for Streptococcus sanguinis, Streptococcus parasanguinis, and Streptococcus gordonii. We designed the S. sanguinis-, S. parasanguinis-, and S....
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Detection of a Familial Y/l5 Translocation by FISH, G-Banding, C-Banding, and Ag-NOR Stain

Lee KM, Kim KH, Han JY, Je GH, Shaffer LG

  • KMID: 2146218
  • Korean J Clin Pathol.
  • 1997 Dec;17(6):1176-1181.
Prenatal chromosome analysis of amniotic cells at 18 weeks of gestation showed a male fetus to carry a large 15p+ derivative chromosome inherited from his mother. Extra genetic material on...
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Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes

Park CH, Kim PS, Kim HS, Min JB, Hwang HK, Jang HS, Cho KW, Baek DH, Kook JK

  • KMID: 1719928
  • Int J Oral Biol.
  • 2010 Mar;35(1):13-19.
The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization....
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Proposal of an Appropriate Decalcification Method of Bone Marrow Biopsy Specimens in the Era of Expanding Genetic Molecular Study

Choi SE, Hong SW, Yoon SO

BACKGROUND: The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A...
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Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections

Kim O

  • KMID: 1094690
  • J Vet Sci.
  • 2004 Mar;5(1):71-73.
Two non-radioactive probes using digoxigenin or biotin were developed for detecting canine herpesvirus (CHV) and compared for their sensitivities by in situ hybridization (ISH) in formalin fixed, paraffin embedded sections,...
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Identification of Human Papillomavirus(HPV) in Patients with Cervical Cancer by DNA Hybridization

Song SK, Ryu KS

  • KMID: 2245190
  • Korean J Gynecol Oncol Colposc.
  • 1990 Mar;1(1):72-79.
Genomic DNAs were extracted from cervical lavages of 49 patients with cervical cancer. Dot and Southern blot hybridization were performed using the P-labeled HFV DNA probes to find high risk...
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Primary Squamous Cell Carcinoma of the Upper Genital Tract: Utility of p16INK4a Expression and HPV DNA Status in its Differential Diagnosis from Extended Cervical Squamous Cell Carcinoma

Yoo SH, Son EM, Sung CO, Kim KR

  • KMID: 2012156
  • Korean J Pathol.
  • 2013 Dec;47(6):549-556.
BACKGROUND: Primary squamous cell carcinoma (SCC) of the upper genital tract, including the endometrium, fallopian tubes, and ovaries, is extremely rare. It must be distinguished from the mucosal extension of...
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A Case of Sex-Reversed Male

Nam YS, Kim HJ, Lee SH, Kwak IP, Yoon TK, Cha KY

  • KMID: 2261125
  • Korean J Obstet Gynecol.
  • 1998 Aug;41(8):2216-2219.
The incidence of XX male reversal is approximately 1 in 20,000 to 1 in 24,000 male births, a rare disorder. The affected individuals are non-ambiguous at birth with normal male...
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Prevalence of Seasonal Influenza Viruses and Pandemic H1N1 Virus in Beijing from 2008 to 2012

Cui S, Tian L, Peng X, Lu G, Shi W, Meng D, Wang Q

  • KMID: 2434492
  • Ann Lab Med.
  • 2012 Nov;32(6):455-456.
No abstract available.
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Profiling of Differentially Expressed Genes in Human Polymorphonuclear Leukocyte on Human Amniotic Membrane

Kim HK, Han GH, Choi TH

  • KMID: 2337120
  • J Korean Ophthalmol Soc.
  • 2003 Nov;44(11):2615-2626.
PURPOSE: To identify genes that showed altered expression between human polymorphonuclear (PMN) cell cultured on plastic and on amniotic membrane by the technique of differential hybridization of two Altas(TM) Human...
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Detection Limit of Minimal Residual Disease in Leukemia Using Fluorescence in situ Hybridization

Lee JH, Kim SY, Kim HH, Suh BK, Lee WB, Whang KT

  • KMID: 1967595
  • J Korean Pediatr Soc.
  • 2000 Oct;43(10):1364-1371.
PURPOSE: The close relationship between the size of a tumor burden and the curability of acute leukemia is well established. Therefore, it is very irnportant to detect residual leukemia accurately at low...
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Detection and Quantification of Residual Cellular DNA in the Production of Recombinant HPV-16 L1 Virus-Like Particles

Son HK, Jeong HS, Kim YL, Kim SN, Shin JH, Min HK, Seong BL, Park SN

A number of recombinant proteins isolated from cell sources are being produced for biopharmaceuticals. Although most biopharmaceuticals are highly purified, there is a safety concern that such recombinant products could...
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A case of Prader-Willi syndrome with bilateral cryptorchidism and diabets mellitus

Jung HS, Shim MS, Kim MJ, Kim SH, Yoon SJ, Jang KW, Jang IY, Lee BJ, Yang YH, Kim SJ, Shin YG, Chung CH

  • KMID: 2306110
  • Korean J Med.
  • 2002 Oct;63(4):426-430.
Prader-Willi syndrome (PWS) is a complex, multisystem disorder comprising congenital hypotonia, feeding difficulties, hypogonadism and hypogenitalism, short stature, small hands and feet, mental and psychomotor retardation, distinctive facial appearance, onset...
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Chromosome Analysis in Clinical Samples by Chromosome Diagnostic System Using Fluorescence in Situ Hybridization

Moon SY, Pang MG, Oh SK, Ryu BY, Hwang D, Jung BJ, Choe J, Sohn C, Chang JK, Kim JW, Kim SH, Choi YM

  • KMID: 2235766
  • Korean J Fertil Steril.
  • 1997 Dec;24(3):335-340.
Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive...
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Analysis of Chromosome-specific Aneusomy in Breast Tumor by Using Fluorescence in Situ Hybridization

Kang SS, Lee HK, Park BJ, Lee JH, Kim JM, Kim YM, Choi SK, Yoon SO, Lee SG

  • KMID: 2004819
  • J Korean Surg Soc.
  • 2000 May;58(5):599-606.
PURPOSE: Several studies have used FISH (fluorescence in situ hybridization) to analyze aneuploids in various solid tumors. FISH, using chromosome-specific, alpha-stellite DNA probes, can be used to detect aneusomy in...
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Evaluation of Self-collected Pad Sampling for the Detection of HPV In Cervicovaginal Secretion

Kim SR, Song SY, Kim DS, Lee JW, Park CS, Bae DS, Lee HJ, Kim KT, Kwon OJ, Song ES, Joo HJ, Ahn G

  • KMID: 2275475
  • Korean J Pathol.
  • 2004 Aug;38(4):258-264.
BACKGROUND: Self-collection of secretion samples for HPV testing is a feasible alternative method for women who would decline to participate in population based cervical cancer programs. The purpose of this...
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