- Development of epidemiological method for the Helicobacter pylori by polymerase chain reaction
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Lee WK, Cho MJ, Choi HJ, Rhee KH
- KMID: 1421876
- J Korean Med Sci.
- 1991 Dec;6(4):338-347.
- doi: 10.3346/jkms.1991.6.4.338
The polymerase chain reaction was used to develop a method for the detection of Helicobacter pylori, a causative agent of gastritis, as well as for the elucidation of its mode... -
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- High Prevalence of Multiple Strain Colonization of Helicobacter pylori in Korean Patients: DNA Diversity Among Clinical Isolates from the Gastric Corpus, Antrum and Duodenum
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Kim JW, Kim JG, Chae SL, Cha YJ, Park SM
- KMID: 759677
- Korean J Intern Med.
- 2004 Mar;19(1):1-9.
BACKGROUND: The aims of our study were to determine the correlation of the strain variation and degree of homogeneity of infecting Helicobacter pylori (H. pylori) with their disease outcomes, and... -
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- Differentiation of Mycobacterium tuberculosis strains by arbitrarily primed polymerase chain reaction-based DNA fingerprinting
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Lee TY, Lee TJ, Kim SK
- KMID: 1728913
- Yonsei Med J.
- 1994 Sep;35(3):286-294.
- doi: 10.3349/ymj.1994.35.3.286
Arbitrarily primed polymerase chain reaction (AP-PCR) method was applied to the differentiation of clinical isolates of M. tuberculosis strains. The primer which is specific to human papilloma virus (HPV) type... -
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- Molecular Typing in Public Health Laboratories: From an Academic Indulgence to an Infection Control Imperative
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Allerberger F
- KMID: 1120117
- J Prev Med Public Health.
- 2012 Jan;45(1):1-7.
- doi: 10.3961/jpmph.2012.45.1.1
Using three Austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on DNA subtyping. DNA macrorestriction... -
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- Random amplified polymorphic DNA (RAPD) analysis of Mycobacterium tuberculosis strains in India
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Singh JP, Verma R, Chaudhuri
- KMID: 1059206
- J Vet Sci.
- 2006 Jun;7(2):181-187.
- doi: 10.4142/jvs.2006.7.2.181
The usefulness of random amplification of polymorphic DNA (RAPD) analysis for typing Indian strains of M. tuberculosis was investigated. M. tuberculosis H37Rv, M. tuberculosis DT and 42 clinical isolates of M. tuberculosis were subjected... -
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- Diagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids
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Cho CH, Han SH, Chin BS, Choi SH, Lee HS, Kim CO, Kim MS, Choi JY, Song YG, Kim JM
- KMID: 1717964
- Korean J Intern Med.
- 2009 Jun;24(2):135-138.
- doi: 10.3904/kjim.2009.24.2.135
BACKGROUND/AIMS: The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid... -
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- Evaluation of DNA Extraction Methods and Their Clinical Application for Direct Detection of Causative Bacteria in Continuous Ambulatory Peritoneal Dialysis Culture Fluids from Patients with Peritonitis by Using Broad-Range PCR
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Kim SH, Jeong HS, Kim YH, Song SA, Lee JY, Oh SH, Kim HR, Lee JN, Kho WG, Shin JH
- KMID: 1245251
- Ann Lab Med.
- 2012 Mar;32(2):119-125.
- doi: 10.3343/alm.2012.32.2.119
BACKGROUND: The aims of this study were to compare several DNA extraction methods and 16S rDNA primers and to evaluate the clinical utility of broad-range PCR in continuous ambulatory peritoneal... -
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- Detection of Bartonella henselae DNA by Polymerase Chain Reaction in a Patient with Cat Scratch Disease: A Case Report
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Chung JY, Han TH, Kim BN, Yoo YS, Lim SJ
- KMID: 1781778
- J Korean Med Sci.
- 2005 Oct;20(5):888-891.
- doi: 10.3346/jkms.2005.20.5.888
We report a case of cat scratch disease caused by Bartonella henselae in Korea. A 25-yr-old woman developed left cervical lymphadenopathy with history of contact with a dog. The cervical... -
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- Evaluation of Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile Assays for Direct Detection of Toxigenic Clostridium difficile in Stool Specimens
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Shin BM, Yoo SM, Shin WC
- KMID: 2373512
- Ann Lab Med.
- 2016 Mar;36(2):131-137.
- doi: 10.3343/alm.2016.36.2.131
BACKGROUND: We evaluated the performance of four commercial nucleic acid amplification tests (NAATs: Xpert C. difficile, BD MAX Cdiff, IMDx C. difficile for Abbott m2000, and Illumigene C. difficile) for... -
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- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based VITEK MS System for the Identification of Acinetobacter Species from Blood Cultures: Comparison with VITEK 2 and MicroScan Systems
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Lee SY, Shin JH, Kim SH, Shin MG, Suh SP, Ryang DW
- KMID: 2363149
- Ann Lab Med.
- 2015 Jan;35(1):62-68.
- doi: 10.3343/alm.2015.35.1.62
BACKGROUND: Acinetobacter species are the leading cause of bloodstream infection (BSI), but their correct identification is challenging. We evaluated the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based... -
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- Evaluation of a Real-Time PCR Assay for Simultaneous Detection of Kingella kingae and Staphylococcus aureus from Synovial Fluid in Suspected Septic Arthritis
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Haldar M, Butler M, Quinn CD, Stratton C, Tang YW, Burnham CA
- KMID: 1791940
- Ann Lab Med.
- 2014 Jul;34(4):313-316.
- doi: 10.3343/alm.2014.34.4.313
Direct plating of synovial fluid (SF) on agar-based media often fails to identify pathogens in septic arthritis (SA). We developed a PCR assay for the simultaneous detection of Kingella kingae... -
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- Methicillin-resistant Staphylococcus aureus in Nasal Surveillance Swabs at an Intensive Care Unit: An Evaluation of the LightCycler MRSA Advanced Test
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Huh HJ, Kim ES, Chae SL
- KMID: 2434481
- Ann Lab Med.
- 2012 Nov;32(6):407-412.
BACKGROUND: We compared the LightCycler MRSA advanced test (Roche Diagnostics, Germany) with enrichment culture methods to evaluate the relative diagnostic performance of the LightCycler MRSA advanced test for active surveillance... -
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- Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
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Kim YJ, Lee SM, Park BK, Kim SS, Yi J, Kim HH, Lee EY, Chang CL
- KMID: 1791921
- Ann Lab Med.
- 2014 May;34(3):203-209.
- doi: 10.3343/alm.2014.34.3.203
BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether... -
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- Evaluation of Vancomycin Resistance 3 Multiplexed PCR Assay for Detection of Vancomycin-Resistant Enterococci from Rectal Swabs
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Cekin Y, Erman Daloglu A, Ogunc D, Ozhak Baysan B, Daglar D, Inan D, Mutlu D, Ongut G, Colak D
- KMID: 1781337
- Ann Lab Med.
- 2013 Sep;33(5):326-330.
- doi: 10.3343/alm.2013.33.5.326
BACKGROUND: Active screening for vancomycin-resistant enterococci (VRE) using rectal specimens is recommended to limit the spread of antimicrobial resistance within certain high-risk populations. We evaluated the diagnostic performance of Vancomycin... -
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- Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods
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Lee H, Park KG, Lee G, Park J, Park YG, Park YJ
- KMID: 1781360
- Ann Lab Med.
- 2014 Jan;34(1):51-55.
- doi: 10.3343/alm.2014.34.1.51
Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM... -
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- Application of PCR from the fine needle aspirates for the diagnosis of cervical tuberculous lymphadenitis
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Kim SS, Chung SM, Kim JN, Lee MA, Ha EH
- KMID: 644939
- J Korean Med Sci.
- 1996 Apr;11(2):127-132.
- doi: 10.3346/jkms.1996.11.2.127
Tuberculosis remains a major public health problem worldwide. A definitive and accurate diagnosis of tuberculosis in cervical lymphadenopathy is important because satisfactory results can be achieved with chemotherapy alone, obviating... -
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- Application of HTB-SiHa Cells Transfected with a Recombinant Plasmid for External Quality Assessment of Chlamydia trachomatis PCR
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Zhang K, Huo H, Sun Y, Wang L, Zhang R, Lin G, Xie J, Wang Q, Li J
- KMID: 1791950
- Ann Lab Med.
- 2014 Sep;34(5):360-366.
- doi: 10.3343/alm.2014.34.5.360
BACKGROUND: The participation of laboratories in external quality assessment (EQA) programs is required for the quality assurance of nucleic acid amplification of Chlamydia trachomatis. This study aimed to construct a... -
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- Usefulness of a Rapid Real-time PCR Assay in Prenatal Screening for Group B Streptococcus Colonization
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Park JS, Cho DH, Yang JH, Kim MY, Shin SM, Kim EC, Park SS, Seong MW
- KMID: 1781296
- Ann Lab Med.
- 2013 Jan;33(1):39-44.
- doi: 10.3343/alm.2013.33.1.39
BACKGROUND: Group B streptococcus (GBS) infection is a leading cause of neonatal morbidity and mortality worldwide. Here, we present the analytical and diagnostic usefulness of a new real-time PCR-based assay... -
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- Epidemiological Aspects of Pertussis among Adults and Adolescents in a Korean Outpatient Setting: A Multicenter, PCR-Based Study
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Park S, Lee SH, Seo KH, Shin KC, Park YB, Lee MG, Yoo KH, Kim HJ, Park JS, Cho JH, Ko Y, Lee SK, Cheon KT, Kim DI, Ha JW, Lee JM, Suhr JW, Jeong EH, Jung KS
- KMID: 1794601
- J Korean Med Sci.
- 2014 Sep;29(9):1232-1239.
- doi: 10.3346/jkms.2014.29.9.1232
Epidemiological data of Bordetella pertussis infection among adolescents and adults are limited in Korea. Patients (> or = 11 yr of age) with a bothersome cough for less than 30... -
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- Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens
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Choi YJ, Kim HJ, Shin HB, Nam HS, Lee SH, Park JS, Park KS, Baek KA
- KMID: 1380084
- Ann Lab Med.
- 2012 Jul;32(4):257-263.
- doi: 10.3343/alm.2012.32.4.257
BACKGROUND: A peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR(TM) TB/NTM detection kit; PANAGENE, Korea) assay has been recently developed for the simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and... -
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