- Purification and Characterization of an Extracellular Alkaline Protease from Aspergillus niger C-15
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Kim JD
- KMID: 1516938
- Mycobiology.
- 2004 Jun;32(2):74-78.
- doi: 10.4489/MYCO.2004.32.2.074
An alkaline protease produced by Aspergillus niger C-15 was purified and characterized. The enzyme was purified 19.41-fold with a specific activity of 74150 U/mg and a recovery of 34.4% by... -
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- Purification and Characterization of Intracellular Cellulase from Aspergillus oryzae ITCC-4857.01
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Begum MF, Absar N
- KMID: 2408782
- Mycobiology.
- 2009 Jun;37(2):121-127.
Purification and characterization of intracellular cellulase produced by A. oryzae ITCC-4857.01 are reported. The enzyme was purified by ion-exchange chromatography using DEAE-cellulose followed by Gel filtration. The purification achieved was... -
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- An Emerging Diabetes Mellitus Diagnosis Modality: HbA1c
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Seo HA, Lee IK
- KMID: 1776563
- Korean J Intern Med.
- 2012 Mar;27(1):39-40.
- doi: 10.3904/kjim.2012.27.1.39
No abstract available. -
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- Isolation and Identification of the Antigen Recognized by Human Cytomegalovirus Specific Monoclonal Antibody SCMVM 34
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Park CG, Kook YH, Cha CY, Hwang ES, Lim DG, Seoh JY, Park JW, Jong HS
- KMID: 1540528
- J Korean Soc Microbiol.
- 1997 Jun;32(3):325-334.
Human cytomegalovirus (HCMV)-specific monoclonal antibody, SCMVM 34, recognizes early antigen confined to the nucleus of HCMV-infected cells. This study was performed to identify the antigen reactive to SCMVM 34 with... -
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- Binding of B Cell - Derived Autocrine Growth Factor to Hemoglobin
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Park JS, Choi IW, Shin YM
- KMID: 2083011
- Korean J Immunol.
- 1998 Mar;20(1):25-30.
Normal human B cells produce autocrine growth factor in response to Staphylococcus aureus Cowan I strain (SAC). However, the functional role and molecular nature of the B cell derived-B cell... -
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- Effects of Sample Pretreatment in Amino Acid Analysis
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Kim MH, Moon HR
- KMID: 2300485
- Korean J Clin Pathol.
- 2001 Feb;21(1):34-39.
BACKGROUND: Measurements of the concentrations of free amino acids in the blood are used as useful biochemical indicators. The sample pretreatments, including anticoagulant selection and deproteinization, are important steps in... -
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- Analysis of the Chemical Constituents of Agaricus brasiliensis
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Cho SM, Jang KY, Park HJ, Park JS
- KMID: 2052888
- Mycobiology.
- 2008 Mar;36(1):50-54.
- doi: 10.4489/MYCO.2008.36.1.050
This study examined the chemical composition of A. blasiliensis and the chemical structural properties of an immuno-stimulating polysaccharide. The amino acids, free sugars, and organic acids by HPLC and fatty... -
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- Molecular pharmacological interaction of phenylbutazone to human neutrophil elastase
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Kang K
- KMID: 1849847
- Korean J Physiol Pharmacol.
- 1998 Jun;2(3):385-393.
Human neutrophil elastase (HNElastase, EC 3.4.21.37), a causative factor of inflammatory diseases, was purified by Ultrogel AcA54 gel filtration and CM-Sephadex ion exchange chromatography. HNElastase was inhibited by phenylbutazone in a concentration dependent... -
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- Antioxidant Assay of Extracted Fractions Xanthium Strumarium L Using Lens Protein Crosslink Activity
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Lee SJ, Lee KW, Chung YS, Hong EK, Lee JH, Wee WR, Joo CK
- KMID: 2109323
- J Korean Ophthalmol Soc.
- 2002 Apr;43(4):751-756.
PURPOSE: To elucidate the anti-oxidant effect of extract fractions from Xanthium strumarium L. on lens protein by crosslinking assay. METHODS: [(1 4)C] N-formyl-lysine was synthesized and purified by ion exchange chromatography.... -
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- Production of Monoclonal Antibody to Polychlorinated Biphenyl Induced Cytochrome P-450 LMII in Rat Liver
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Kim JH, Kim JR, Lee KY
- KMID: 2373871
- Yeungnam Univ J Med.
- 1986 Dec;3(1):103-110.
- doi: 10.12701/yujm.1986.3.1.103
Cytochrome P-450 (CP-450) is one of the three components of the liver microsomal enzyme system which hydroxylates fatty acids, hydrocarbons and a variety of drugs and other foreign compounds. Female... -
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- Purification and Characterization of a Keratinase from a Feather-Degrading Fungus, Aspergillus flavus Strain K-03
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Kim JD
- KMID: 2312534
- Mycobiology.
- 2007 Dec;35(4):219-225.
A keratinolytic enzyme secreted by Aspergillus flavus K-03 cultured in feather meal basal medium (FMBM) containing 2% (w/v) chicken feather was purified and characterized. Keratinolytic enzyme secretion was the maximal... -
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- Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves
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Varshney B, Ponnanna N, Sarkar PA, Rehman P, Shah JH
- KMID: 1104237
- J Vet Sci.
- 2007 Mar;8(1):57-64.
- doi: 10.4142/jvs.2007.8.1.57
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies... -
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- Isolation of a Specific Antigen from the Triton X-100 Soluble Protein (TSP) Of Mycobacterium tuberculosis
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Paik TH, Kwon HS, Lee S, Lee JS, Jo EK, Kim HJ, Lee M, Yoo YC, Park JK
- KMID: 1579120
- J Bacteriol Virol.
- 2004 Dec;34(4):273-282.
Tremendous efforts have been made to develop better vaccines and diagnostic markers for the effective control of tuberculosis. Recently, we reported that the Triton X-100 soluble protein (TSP) of Mycobacterium... -
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- Isolation and characterization of brain-specific transglutaminases from rat
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Kwak SJ, Kim SY, Kim YS, Song KY, Kim IG, Park SC
- KMID: 755006
- Exp Mol Med.
- 1998 Dec;30(4):177-185.
The relevance of transglutaminases with neural function and several disorders has been emphasized recently. Especially, many polypeptides associated with neurodegenerative diseases are suggested to be putative transglutaminase substrates such as... -
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- Enzymatic N-glycan analysis of 31 kDa molecule in plerocercoid of Spirometra mansoni (sparganum) and its antigenicity after chemical oxidation
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Chung YB, Kong Y, Yang HJ
- KMID: 1051419
- Korean J Parasitol.
- 2004 Jun;42(2):57-60.
- doi: 10.3347/kjp.2004.42.2.57
A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein... -
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- Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenesis
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Lee OR, Chung PR
- KMID: 1039034
- Korean J Parasitol.
- 2001 Jun;39(2):119-132.
- doi: 10.3347/kjp.2001.39.2.119
An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of D1 fraction (D1A) among 5 antigenic protein fractions partially purified by DEAE-anion exchange chromatography from water-soluble... -
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- Partial characterization of a 29 kDa cysteine protease purified from Taenia solium metacestodes
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Kim JY, Yang HJ, Kim KS, Chung YB
- KMID: 1729473
- Korean J Parasitol.
- 2005 Dec;43(4):157-160.
- doi: 10.3347/kjp.2005.43.4.157
A 29 kDa cysteine protease of Taenia solium metacestodes was purified by Mono Q anion-exchanger and Superose 6 HR gel filtration chromatography. The enzyme was effectively inhibited by cysteine protease... -
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- The Correlation of Plasma Homocysteine and Mitochondrial DNA Content in Peripheral Blood in Healthy Women
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Lim S, Shin WS, Park KS, Kim SY, Lee JH, Yim MJ, Song JH, Lee HK
- KMID: 2199618
- J Korean Soc Endocrinol.
- 2000 Jun;15(2):248-261.
BACKGROUND: Hyperhomocysteinemia is an independent risk factor for cardiovascular diseases. Previous reports showed that homocysteine damages mitochondrial gene expression, function and structure. In recent years, homocysteine and mitochondrial DNA (mtDNA)... -
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- Partial Purification and Properties of a Cysteine Protease from Citrus Red Mite Panonychus citri
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Hong SC, Her KH, Kim HU, Lee J, Lee SP, Chung YB
- KMID: 1715683
- Korean J Parasitol.
- 2014 Feb;52(1):117-120.
- doi: 10.3347/kjp.2014.52.1.117
Several studies have reported that the citrus red mites Panonychus citri were an important allergen of citrus-cultivating farmers in Jeju Island. The aim of the present study was to purify... -
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- Validity of Glycated Hemoglobin in Screening and Diagnosing Type 2 Diabetes Mellitus in Chinese Subjects
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Yu Y, Ouyang XJ, Lou QL, Gu LB, Mo YZ, Ko GT, Chow CC, So WY, Ma R, Kong A, Brown N, Nan J, Chan J, Bian RW
- KMID: 1776564
- Korean J Intern Med.
- 2012 Mar;27(1):41-46.
- doi: 10.3904/kjim.2012.27.1.41
BACKGROUND/AIMS: The application of glycated hemoglobin (HbA1c) for the diagnosis of diabetes is currently under extensive discussion. In this study, we explored the validity of using HbA1c as a screening... -
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